Changes in B cells in response to PRRSv infection in pregnant gilts. A) Dot plots: The gating strategy for B cells (CD79α+) and CD21-defined subpopulations is demonstrated using representative data from gilt #53. B-D) Line charts: Changes in absolute numbers (mean ± SD) of total B cells (turquois), CD21− (purple), and CD21+ (pink) B cells are presented from 111 INOC and 19 CTRL gilts over time. P-values indicate significant differences between INOC and CTRL gilts on individual days. E) Bar chart: The mean percentages (+SD) of B cells expressing CD21 within total CD79α+ B cells from INOC and CTRL gilts are presented for the respective study days. Superscript letters indicate significant differences (P < 0.01) between study days within INOC or CTRL gilts.