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Figure 1 | Veterinary Research

Figure 1

From: The innate immune response of equine bronchial epithelial cells is altered by training

Figure 1

Characterization of equine bronchial epithelial cells (EBEC). (A) Morphological appearance of EBEC cultures 7 days after plating (phase contrast microscopy; objective magnification: x4). The biopsy is situated on the left bottom corner of the image. The biopsy is surrounded by growing EBEC. Note the polygonal shape of EBEC. (B) Relative mRNA expression of Fibroblast growth factor-1 (FGF-1) and Fibroblast specific protein-1 (FSP-1) in EBEC and fibroblasts. Significant differences (P < 0.05) in mRNA expression are identified with * for differences between EBEC and fibroblasts. (C) Relative mRNA expression of E-cadherin and Keratin19 in EBEC and fibroblasts. Significant differences (P < 0.05) in mRNA expression are identified with * for differences between EBEC and fibroblasts. (D) Flow cytometry analysis of in vitro cultured EBEC and fibroblasts for the epithelial cell marker cytokeratin and the mesenchymal cell marker vimentin. (C-D) EBEC were assessed at their first passage (4–6 weeks of culture). Fibroblasts were assessed at their first passage (7–10 days of culture). The phenotype and purity of EBEC were maintained during the study.

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