Growth, extracellular proteolytic activity and colony spreading of
wild-type and fpgA
(A) Growth curve at 12 °C in NBF medium, monitored by determining OD525. (■) wild-type and (●) fpgA− strains. Extracellular caseinolytic activity in cell-free supernatants was determined with azocasein as described by Secades et al. . (□) wild-type and (○) fpgA− strains. EU, enzyme units. (B) Colony spreading of F. psychrophilum strains grown on 1/6NA for 96 h at 20 °C according to Pérez-Pascual et al. . (a) wild-type; (b) fpgA−; and (c) fpgA+ (complemented strain).