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Table 1 Primers used for SNP identification of fTNFA and fCD209 gene

From: Polymorphisms in the feline TNFA and CD209 genes are associated with the outcome of feline coronavirus infection

Target gene/region

Positiona

Orientationb

Sequence (5′ - 3′)

TAc

Amplicon size

fTNFA/5′-PRRd

−847 to −827

F

GAATTCCCAGGGTTGCTTTCA

65 °C

1018 bp

 

+171 to +153

R

GCCGATCACTCCAAAGTGC

  

fCD209/5′-PRR

−1057 to −1038

F

GAAGCGGGCTTCTTGTTGAC

65 °C

1076 bp

 

+19 to +1

R

GCTCCTTGGGGTCACACAT

  

fCD209/ECDe

+1818 to +1838

F

CCAAGATCTGATGCATCTGCT

67 °C

1350 bp

 

+3168 to +3149

R

ATGAGCTCGTTGCCTGATCT

  
  1. aThe nucleotide positions are numerated from the translation start point (+1).
  2. bF: forward; R: reverse.
  3. cAnnealing temperature.
  4. d5′-proximal regulatory region.
  5. eExtracellular domain.
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Veterinary Research

ISSN: 1297-9716