Serology and HEV RNA detection. Group 1: Intravenous inoculation of wild boar. Group 2: Intravenous inoculation of miniature pigs. Group 3: Contact infection of wild boar and miniature pigs. A) Antibody responses to HEV in serum of inoculated wild boar and miniature pigs measured by a double-antigen sandwich ELISA. OD450-values ≥1 are prescribed as seropositive – this threshold is indicated as a grey-dashed line. B) HEV RNA in serum and faeces of HEV inoculated wild boar and miniature pigs estimated by RT-qPCR. * Sudden death at 1 dpi (after blood collection).