Single Trichinella larva isolated from Dutch red fox. A. One larva was isolated from a fox carcass that had been frozen at −80 °C for one week. Note the retracted granular inner structure of the larva. No PCR product could be generated from this specimen. B. Dead (unfrozen) comma shaped T. spiralis larva. Original magnification 46×, Olympus BH-2 microscope, maximum contrast settings), bars represent 100 μm.