Table 1 Primer and probe sequences for quantitative reverse transcription PCR assays
Gene | GenBank acc. No | Primer sequence | Probe sequence |
|---|---|---|---|
Chicken | |||
18S rRNA | AF173612.1 | Fwd :TGTGCCGCTAGAGGTGAAATT | 5′ (6FAM) TTGGACCGGCGCAAGACGAAC 3′ (TAMRA) |
Rev: TGGCAAATGCTTTCGCTTT | |||
IL-6 | EU170468 | Fwd :CACGATCCGGCAGATGGT | 5′ (6FAM)ATAAATCCCGATGAAGTGGTCATCC 3′ (TAMRA) |
Rev: TGGGCGGCCGAGTCT | |||
IL-8 /CXCLi1(K60) | NM_205018.1 | Fwd :CCCTCGCCACAGAACCAA | 5′ (6FAM)CCCAGGTGACACCCGGAAGAAACA 3′ (TAMRA) |
Rev: CAGCCTTGCCCATCATCTTT | |||
IFN-α | EU367971 | Fwd :CTTCCTCCAAGACAACGATTACAG | 5′ (6FAM)CCTGCGCCTGGGAACACGTCC 3′ (TAMRA) |
Rev: AGGAACCAGGCACGAGCTT | |||
LITAF | AY765397 | Fwd :CCCTTCTGAGGCATTTGGAA | Â |
Rev: CAGCCTGCAAATTTTGTCTTCTT | |||
STAT-3 | NM_001030931.1 | Fwd: TGGGTGGAGAAGGACATCA | Â |
Rev: CATGGGCAGGTCAATGGTAT | |||
Duck | |||
Duck IL-6 | AB191038 | Fwd :CCAAGGTGACGGAGGAAGAC | 5′ (6FAM)TGTCTCCTGGCTGGCTTCGACGA 3′ (TAMRA) |
Rev: TGGAGAGTTTCTTCAAGCATTTCTC | |||
Duck IL-8 | AB236334.1 | Fwd :AGCCTGGTAAGGATGGGAAAC | 5′ (6FAM)AGCTCCGGTGCCAGTGCATAAGCA 3′ (TAMRA) |
Rev: GGGTGGATGAACTTCGAGTGA | |||
Duck IFN-α | DQ861429 | Fwd :AACCAGCTTCAGCACCACATC | 5′ (6FAM)TGCTTCCCAGCCGACGCC 3′ (TAMRA) |
Rev: TGTGGTTCTGGAGGAAGTGTTG | |||