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Table 1 Vaccination schemes of the four studies

From: Immunogenicity and efficacy of fowlpox-vectored and inactivated avian influenza vaccines alone or in a prime-boost schedule in chickens with maternal antibodies

Study

Chickens

1stvaccination at day 0

2ndvaccination at day 21 (studies 1 and 2) or at day 14 (studies 3 and 4)

Challenge

Vaccine

Dose

Vaccine

Dose

1

SPF

vFP2211

103 TCID50

-

-

No

H5N9 + FP MDA

vFP2211

103 TCID50

-

-

vFP2211

105 TCID50

-

-

vFP2211

105 TCID50

inH5N9

0.5 mL

-

-

inH5N9

0.5 mL

2

H5N9 + FP MDA

inH5N9

0.2 mL

inH5N9

0.5 mL

No

vFP89

103 TCID50

inH5N9

0.5 mL

SPF

vFP89

103 TCID50

inH5N9

0.5 mL

3

SPF

vFP89

103 TCID50

inH5N9

0.3 mL

No

-

-

inH5N9

0.3 mL

H5N9 MDA

vFP89

103 TCID50

inH5N9

0.3 mL

-

-

inH5N9

0.3 mL

FP

vFP89

103 TCID50

inH5N9

0.3 mL

-

-

inH5N9

0.3 mL

H5(N8)* + H5N9 + FP MDA

vFP89

103 TCID50

inH5N9

0.3 mL

4

SPF

-

-

-

-

Yes (D28)

vFP89

103 TCID50

-

-

FP MDA

vFP89

103 TCID50

inH5N9

0.3 mL

-

-

inH5N9

0.3 mL

H5N9 MDA

-

-

-

-

vFP89

103 TCID50

-

-

vFP89

103 TCID50

inH5N9

0.3 mL

-

-

inH5N9

0.3 mL

  1. Chickens were hatched from unvaccinated (SPF) or vaccinated (MDA) hens. Chicks with H5N9, FP, H5N8 and/or H5N1 MDA were the progeny of hens vaccinated with inH5N9, fowlpox (FP), vFP89 (HA gene from A/turkey/Ireland/1378/83 [H5N8]) and/or vFP2211 (HA gene from A/chicken/Indonesia/7/2003 [H5N1]), respectively. The groups of 9 to 10 one-day-old birds received either vFP2211 or vFP89 or inH5N9 at D0, followed by a boost of inH5N9 at D21 (studies 1and 2) or at D14 (studies 3 and 4). In addition, a HPAI H5N1 challenge was performed in study 4.
  2. *H5(N8) MDA are directed against the HA only of A/turkey/Ireland/1378/83 [H5N8]. Breeders of these chicks were indeed vaccinated with a fowlpox expressing the HA of H5N8 (vFP89).