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Table 1 Vaccination schemes of the four studies

From: Immunogenicity and efficacy of fowlpox-vectored and inactivated avian influenza vaccines alone or in a prime-boost schedule in chickens with maternal antibodies

Study Chickens 1stvaccination at day 0 2ndvaccination at day 21 (studies 1 and 2) or at day 14 (studies 3 and 4) Challenge
Vaccine Dose Vaccine Dose
1 SPF vFP2211 103 TCID50 - - No
H5N9 + FP MDA vFP2211 103 TCID50 - -
vFP2211 105 TCID50 - -
vFP2211 105 TCID50 inH5N9 0.5 mL
- - inH5N9 0.5 mL
2 H5N9 + FP MDA inH5N9 0.2 mL inH5N9 0.5 mL No
vFP89 103 TCID50 inH5N9 0.5 mL
SPF vFP89 103 TCID50 inH5N9 0.5 mL
3 SPF vFP89 103 TCID50 inH5N9 0.3 mL No
- - inH5N9 0.3 mL
H5N9 MDA vFP89 103 TCID50 inH5N9 0.3 mL
- - inH5N9 0.3 mL
FP vFP89 103 TCID50 inH5N9 0.3 mL
- - inH5N9 0.3 mL
H5(N8)* + H5N9 + FP MDA vFP89 103 TCID50 inH5N9 0.3 mL
4 SPF - - - - Yes (D28)
vFP89 103 TCID50 - -
FP MDA vFP89 103 TCID50 inH5N9 0.3 mL
- - inH5N9 0.3 mL
H5N9 MDA - - - -
vFP89 103 TCID50 - -
vFP89 103 TCID50 inH5N9 0.3 mL
- - inH5N9 0.3 mL
  1. Chickens were hatched from unvaccinated (SPF) or vaccinated (MDA) hens. Chicks with H5N9, FP, H5N8 and/or H5N1 MDA were the progeny of hens vaccinated with inH5N9, fowlpox (FP), vFP89 (HA gene from A/turkey/Ireland/1378/83 [H5N8]) and/or vFP2211 (HA gene from A/chicken/Indonesia/7/2003 [H5N1]), respectively. The groups of 9 to 10 one-day-old birds received either vFP2211 or vFP89 or inH5N9 at D0, followed by a boost of inH5N9 at D21 (studies 1and 2) or at D14 (studies 3 and 4). In addition, a HPAI H5N1 challenge was performed in study 4.
  2. *H5(N8) MDA are directed against the HA only of A/turkey/Ireland/1378/83 [H5N8]. Breeders of these chicks were indeed vaccinated with a fowlpox expressing the HA of H5N8 (vFP89).