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Figure 3 | Veterinary Research

Figure 3

From: Comparison of innate immune agonists for induction of tracheal antimicrobial peptide gene expression in tracheal epithelial cells of cattle

Figure 3

Effects of Pam3CSK4, FSL-1, lipoteichoic acid and flagellin on tracheal antimicrobial peptide gene expression. Dose- and time-dependent effects were measured for Pam3CSK4 (A,B), a TLR2/1 agonist; FSL-1 (C,D), a TLR2/6 agonist; lipoteichoic acid (LTA) (E,F), a TLR2/2 agonist); and flagellin (G,H), a TLR5 agonist. For the dose-response studies (A,C,E,G), confluent bTEC were stimulated in triplicate with various concentrations of agonist for 16 h. For the time-course studies (B,D,F,H), confluent bTEC were stimulated in triplicate for 4, 8, 16 or 24 h with the doses of agonist shown. Gene expression of TAP relative to that of GAPDH was measured using real-time RT-qPCR. Lipopolysaccharide (LPS, 0.1 μg/mL) was used in all assays as a positive control and standard. *, significantly different from unstimulated cells (P<0.05).

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Veterinary Research

ISSN: 1297-9716