Figure 7
From: Comparative histopathological and immunological study of two field strains of chicken anemia virus
Flow cytometry analysis of CD4 + CD8α-, CD4-CD8α + and CD4 + CD8α + (DP) splenocytes of SPF chickens. (a) Analysis was performed in G1 according to forward/side scatter parameters. Most double positive splenocytes were in R5 according to their CD4 and CD8α surface expression. (b) Mean percentage of splenocyte subpopulations from control (□), CAV-10 (■) and CAV-18 () birds. Cells were triple-labelled with anti-CD3, anti-CD4 and anti-CD8α antibodies conjugated to SPRD, FITC and PE, respectively, and analysed by flow cytometry. The results are expressed as the mean (SD) from each group, n = 6. *p < 0.05 vs. Control.