Table 1 Nucleotide sequence of quantitative real-time PCR primers used in this study
Primer name | Sequence (5′-3′) | Annealing temperature (°C) | Amplicon size (bp) | GeneBank accession no. |
|---|---|---|---|---|
CIRBP F | CATCCCTTGGCTGGCTGTAT | 62 | 169 | JZ713052 |
CIRBP R | GGAAATGAATGGCCGACACAC | |||
CDKN2AIP F | ATGGGCCAACAACGTGTTTC | 56 | 103 | JZ713054 |
CDKN2AIP R | GAAAACAGGGGCATCCTCCA | |||
PTMA-α F | GCCCCTGTAACCTCTCTCCT | 55 | 108 | BT057594 |
PTMA-α R | TGTGTACACGGACATTGGGT | |||
TP RhoA F | GTTGGTGATGGTGCTTGTGG | 57 | 185 | JZ713063 |
TP RhoA R | AGAGGCCGTAGTCTGTCGTA | |||
RPL6 F | ATGGCAGAGGGAGACAAGAA | 56 | 146 | NM_001141697 |
RPL6 R | GGTCTCAGTGGTCTTGGTCT | |||
IgL F | AGTGGAGTCCAGGCTGAAGA | 57 | 119 | AF273017 |
IgL R | AGGGTGGGGACACTGTTACT | |||
MHC-I F | CAGGTGTGCACGTTTTCCAG | 57 | 163 | JZ713068 |
MHC-I R | TTGGTGATGACTGCCTGTGG | |||
Hemoglobin F | CAGTGCTGAATAGGCGTTCTT | 62 | 145 | JZ713069 |
Hemoglobin R | ACACTTCAGCACCTTCGGC | |||
Stanniocalcin F | GCCATGACATCCCCGTTTTG | 57 | 144 | JZ713071 |
Stanniocalcin R | GATGTCAAACCCCACCCACT | |||
Beta-actin F* | ATGGAAGGTGAAATCGCC | 53 | 260 | AF157514 |
Beta-actin R* | TGCCAGATCTTCTCCATG | |||
EF-1α F | AGACAGCAAAAACGACCCCC | 57 | 167 | HF563594 |
EF-1α R | AACGACGGTCGATCTTCTCC |