Table 1 Oligonucleotides used in this study
From: First identification of proteins involved in motility of Mycoplasma gallisepticum
Primer | Sequence (5′ to 3′) | Product (length [bp]) |
|---|---|---|
Ad1B | CTCGTAGACTGCGTACC | LM-PCR (variable) |
Ad2B | GATCGGTACGCAGTCTAC | |
C’gapA5 | ATTAggatccAGTATTCAACGTTTCTAAG | MG gapA (911 bp) |
C’gapA3 | TACGaagcttACCTTAATTATTCAATTTTC | |
HisC-f | GATCCCTCGAGCCCGGGGCATGCCATCATCATCATCATCATTAATAGGG | synthetic 6xHis-tag |
HisC-r | CGCCCTATTAATGATGATGATGATGATGGCATGCCCCGGGCTCGA | |
ISL-f | TATAccgcggATAAAGTCCGTATAATTGTG | IS256L (1365 bp) |
ISL-r | TATAccgcggATAAAGTCCGTATAATTGTG | |
ISR-f | TATAacgcgtGATAAAGTCCGTATAATTGTG | IS256R (1342 bp) |
ISR-r | ATTggcccgAAAATAATAAAGGAAGTGAGTC | |
ISM-mgcF | ATAAggatccTGTTGAAAAGCGCTTAGC | MG mgc2 |
ISM-mgcR | TTAAgcatgcTCTAGGTCCATTTTGTGG | (1001) |
Bgl | TAGACTGCGTACCGATC | LM-PCR |
IS-I | TGTACCGTAAAAGGACTG | products |
IS-N | AAAGGACTGTTATATGGC | (variable) |
mgc2_3 | ACGCAGgaattcATAACAATTATG | MG mgc2 |
mgc2_4 | TTTACAaagcttGTCTTATCTAGG | (894) |
mgc_tga | GAAAGATTACCTCCGAACCATGGTTTTATCCAGTAGTGGG | TGA > TGG |
Xcat5 | TAGATGggatccATGGAGAAAAAAATCACTG | pACYC184 (751) |
Xcat3 | ATAAATggcgccCGCTTATTATCACTTATTC | Cm PO + CmR |