Figure 4
From: First identification of proteins involved in motility of Mycoplasma gallisepticum
Absence of MGC2 protein in gliding mutants. Immunoblot analysis of RCL1 (lane 1), motility mutants T932A (lane 2) and T932C (lane 3), and complemented mutants T932A::p5Hmgc (lane 4) and T932C::p5Hmgc (lane 5) with antibodies specific for MGC2 (A), GapA (B), and CrmA (C). No MGC2 was detected in T932A, and T932C weakly displayed a truncated MGC2′, while RCL1 and complemented mutants produced full-length MGC2. Immunoblot analysis of whole cell lysates of RCL1 and mgc2-complemented mutants T932A::p5Hmgc and T932C::p5Hmgc (D) after tryptic digest for 0 (T0), 40 (T1), and 90 min (T2); M, molecular weight marker.