Gene-expression profiling of calves 6 and 9 months after inoculation with Mycobacterium avium subspecies paratuberculosis

Table 2 qPCR primers used for 9 month gene targets

Target	Primer sequence (5'-3')	Amplicon size	NCBI Accession number
BOLA	GAACTACCTGGAGGGCGAGT	229 bp	ENSBTAG00000019386
BOLA	GGTTTCCACAAGCTCCATGT	229 bp	ENSBTAG00000019386
CCR7	CCCTTCTCGTCATTTTCCAG	158 bp	ENSBTAG00000015133
CCR7	GGAGTACATGATCGGGAGGA	158 bp	ENSBTAG00000015133
IGSF6	TTTCCCAACTCAAAGCAACC	238 bp	ENSBTAG00000018869
IGSF6	TACGCCGAGCACTCTTTTTC	238 bp	ENSBTAG00000018869
IL4R	GTGTGCGTGTCCTGCTACAT	202 bp	ENSBTAG00000001602
IL4R	GTAAACAGGGCAGGAGCTTG	202 bp	ENSBTAG00000001602
TEX261	CACCTACATGATTGGCGTTG	226 bp	ENSBTAG00000002105
TEX261	GAAGAACGCAAACGGGATTA	226 bp	ENSBTAG00000002105

ISSN: 1297-9716