Gene-expression profiling of calves 6 and 9 months after inoculation with Mycobacterium avium subspecies paratuberculosis

Table 1 qPCR primers used for 6 month gene targets

Target	Primer sequence (5'-3')	Amplicon size	Ensembl gene ID
BOLA	ACATGGAGCTTGTGGAGACC	283 bp	ENSBTAG00000002069
BOLA	CTTGCAGCCTGGGTGTAGAT	283 bp	ENSBTAG00000002069
BNBD9-Like	TCTTCCTGGTCCTGTCTGCT	106 bp	ENSBTAG00000047740
BNBD9-Like	ATCTGTCTCGTGCGTCCAG	106 bp	ENSBTAG00000047740
ALOX15	CCACCAAGGATGTGACACTG	251 bp	ENSBTAG00000011990
ALOX15	GTATTCGTAGGGCCAGTCCA	251 bp	ENSBTAG00000011990
ALOX5AP	TTCTCTGCAGCCAAGTTCCT	251 bp	ENSBTAG00000013201
ALOX5AP	GAGAAGGAGAGGGGAGATGG	251 bp	ENSBTAG00000013201
S100A9	GTCACAAATGGAAAGCAGCA	238 bp	ENSBTAG00000006505
S100A9	GGCCACCAGCATAATGAACT	238 bp	ENSBTAG00000006505
GPR77	CCGAAACTGTGCACTCAAGA	222 bp	ENSBTAG00000037735
	GCCGAGAGAATTGTTCTCCA

ISSN: 1297-9716