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Table 1 List of primers used in this study

From: The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2

TCRBV amplifications1

IGHV amplifications2

No.:

Primer:

Sequence:

No.:

Primer:

Sequence

1

VβI

tccatgctcttctgctgtgt

10

FR1-5

gaggagaagctggtggagt

2

VβIV

kgcaycgggstkctctg

11

FR1-3

ctcctgtgtcggctctgga

3

VβVII

ctcascggramcctttgc

12

FR3

tgagaaccgaagacacggc

4

FR3βIa

gactchgchstgtwyytctgtg

13

α-Cμ

gggacgaagatgttcaagac

5

FR3βIb

crgacatctvkrtayytstgtg

14

α-Cδ

gctgggagctgccgagat

6

FR3βIV

gactcsgcygtgtatctctg

15

α-Cγ

ccgtccacgtaccaggagaa

7

FR3βVII

gactcrgccacctacstctg

16

α-Cα

gagcccaggagcaggtct

8

α-Cβ1

tctccgcttccgatggttca

17

α-Cε

gtccggatggtggtgtttg

9

α-Cβ2

gtggtctcacctgctgcag

18

α-JH

tgaggacacgacgacttcaa

  1. 1Following primer pairs (stated in brackets for 1st, 2nd and 3rd PCR respectively) were used for TCRBV amplifications: VβI-VβIII families (1/8, 1/9, 4 + 5/9), VβIV-VβVI families (2/8, 2/9, 6/9) and VβVII family (3/8, 3/9, 7/9). Annealing temperatures (Tm) were 54 °C for 1st PCR, 61 °C for 2nd PCR and 55 °C for 3rd PCR.
  2. 2Following primer pairs (stated in brackets for 1st, 2nd and 3rd PCR respectively) were used for IGHV amplifications: total immunoglobulins (10/18, 11/18, 12/18), IgM (10/13, 11/13, 12/18), IgD (10/14, 11/14, 12/18), IgG (10/15, 11/15, 12/18), IgA (10/16, 11/16, 12/18) and IgE (10/17, 11/17, 12/18). Annealing temperatures (Tm) were 58 °C for 1st and 2nd PCR and 55 °C for 3rd PCR.
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Veterinary Research

ISSN: 1297-9716