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Figure 8 | Veterinary Research

Figure 8

From: The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2

Figure 8

CDR3 analysis of B (IGHV) and αβ T (TCRBV) cell receptor repertoires. Representative CDR3 length analysis (spectratyping) of total cells isolated from the blood and BAL (indicated next to spectrotyping curves by “B” and “L” respectively) of control GF piglets (A, E) and GF piglets infected with PRRSV (B, F), SIV (C, G) or PCV2 (D, H). Analysis of IGHV repertoire (A-D) was done for total immunoglobulins (Ig) and also for IgM, IgG, IgA, IgD and IgE classes. Analysis of TCRBV repertoire (E-H) included amplification for VβI - VβIII families (I), VβIV - VβVI families (IV) and VβVII family (VII). For both IGHV and TCRBV, shared bands or bands of interest are indicated by a dark triangle. Vertical lines indicate the mean CDR3 length in the non-selected repertoire of GF piglets. Lengths of CDR3 are indicated on x-axes and include number of nucleotides (nt) from the 3' end of V segment to the 3' end of J segment. The results are representative of three independent experiments.

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Veterinary Research

ISSN: 1297-9716