Figure 2

Analysis of lymphoid cells and their subpopulations in the TBLN. Cell suspensions isolated from TBLN of control GF piglets and GF piglets infected with PRRSV, SIV or PCV2 (different animal groups depicted on x-axes) were analyzed by flow cytometry for the proportion of αβ T cell subpopulations (A-C) and γδ T cell subpopulations (D-F). The phenotype of individual cell subpopulations is depicted above each graph. Note that the proportion of B cells and their subpopulations and also the proportion of total αβ T, γδ T and NK cells did not differ between experimental groups and therefore these are not shown. Each dot in the graphs represents an individual animal and the mean for all animals in the same group is indicated by a horizontal line. Groups of animals that show a statistically significant difference (p < 0.05) from GF animals are light-grey boxed and those differing even within a group are dark-grey boxed.