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Table 3 Results of PCR, histology and IHC in tissues of foetuses/lambs and relationship with maternal serostatus

From: Neospora caninum infection as a cause of reproductive failure in a sheep flock

Sample1 Year Maternal serostatus Results obtained in tissues from foetuses/lambs4
PCR Histology5 IHC6
N. caninum T. gondii N. caninum T. gondii
N. caninumPP2 T. gondiititre3 Brain Liver Heart Lung Brain Brain lesions Brain Brain
1 2011 72.5 < 16 2/2 na na na - na na na
2 2011 86.3 32 2/2 - 1/2 - - na na na
3 2012 89.9 < 16 2/2 - - - - na na na
4$ 2012 44.0 < 16 3/3 1/3 - - - ** + -
5 2011 65.1 < 16 2/2 2/2 2/2 - - na na na
6 2011 65.1 < 16 2/2 2/2 2/2 - - na na na
7# 2011 79.3 < 16 2/2 na na na - na na na
8 2011 0.0 < 16 - nd nd nd - na na na
9 2011 52.0 < 16 2/2 - - - - na na na
10 2011 94.1 32 - nd nd nd - na na na
11 2011 94.1 32 1/2 - - - - na na na
12 2011 82.1 < 16 3/3 - - - - - + -
13 2011 0.0 < 16 - nd nd nd - * - -
14 2012 1.4 < 16 - nd nd nd - Unrelated - -
15 2012 28.1 16 3/3 na na na - na na na
16a 2012 0.0 16 - nd nd nd - na na na
17a 2012 0.0 < 16 - nd nd nd - na na na
18b 2012 0.0 < 16 - nd nd nd - * - -
19b 2012 0.0 < 16 - nd nd nd - - - -
20 2012 64.6 < 16 3/3 na na na - * + -
21 2012 98.9 < 16 3/3 2/3 3/3 1/3 - ** + -
  1. 11-4: aborted foetus; 5–19: stillborn; 20–21: newborn with neurological disorders; $: In this sample, parasite was also detected in placenta, by PCR and IHC; #All organs were analyzed for the presence of bacteria and viruses causing ovine abortions, and the results were negative; a,b: lambs indicated by the same superscript letter were twins; 2Positivity Percentage (PP) as determined by ELISA (cut off ≥ 7.0%); 3Titre determined by agglutination (cut off ≥ 1:64);
  2. 4Fractions represent the number of positive tissue samples/total number of samples checked by PCR; (−): negative results; na: samples were not available or suitable for analysis; nd: not determined when the target organ (brain) was negative by PCR; 5Histological features were classified according to previous descriptions [21] as unrelated, consistent (*) or characteristic (**) lesions of protozoan infection.