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Table 3 Histological lesions (HE) and presence of antigen (IHC) in tissues of H5N1-inoculated gulls during the course of the experiment

From: Experimental infection of highly pathogenic avian influenza virus H5N1 in black-headed gulls (Chroicocephalus ridibundus)

  Number of birds positive/number of birds examined per day
  2 dpi 4 dpi 5 dpi 6 dpi 7 dpi 12 dpi
Tissue HE1 IHC2 HE IHC HE IHC HE IHC HE IHC HE IHC
Thymus 0/4 2/4 0/5 0/5 0/2 0/2 0/2 0/2 0/1 0/1 0/2 0/2
Adrenal 1/4 1/4 0/5 0/5 0/2 0/2 1/2 1/2 0/1 0/1 0/2 0/2
Lung/Trachea 0/4 3/4 1/5 0/5 1/2 0/2 1/2 0/2 0/1 0/1 0/2 0/2
Air sacs 4/4 4/4 5/5 5/5 2/2 1/2 1/2 1/2 0/1 0/1 1/2 1/2
Pancreas 0/4 0/4 2/5 2/5 1/2 1/2 2/2 2/2 1/1 1/1 1/2 0/2
CNS 0/4 0/4 5/5 5/5 2/2 2/2 2/2 2/2 1/1 1/1 1/2 0/2
  1. 1HE: presence of histological lesions by examination of hematoxylin-and-eosin-stained tissue sections.
  2. 2IHC: presence of viral antigen by examination of tissue sections stained by immunohistochemical technique.