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Table 2 Virus detection in pharyngeal and cloacal swabs of H5N1-inoculated gulls during the course of the experiment

From: Experimental infection of highly pathogenic avian influenza virus H5N1 in black-headed gulls (Chroicocephalus ridibundus)

  

Pharyngeal swab

Cloacal swab

  

RT-PCR

Virus isolation

RT-PCR

Virus isolation

Day

No. tested

No. positive

Ct range

No. positive*

Titre range

No. positive

Ct range

No. positive*

Titre range

1

16

16

19-27

16

1.5-5.5

6

27-32

1

< 0.8-0.8

2

16

16

21-29

15

1.2-3.5

16

22-30

4

1.5-2.8

3

12

11

21-29

10

0.8-4.2

6

28-31

1

< 0.8-0.8

4

12

12

22-30

9

1.8-3.8

7

26-33

2

0.8-2.2

5

7

7

23-32

6

1.8-3.8

6

24-31

3

1.2-2.2

6

5

5

20-34

4

0.8-3.5

5

20-30

2

2.2-2.5

7

3

3

23-28

3

1.5-3.2

3

27-32

0

n.a.

9

2

2

31

0

n.a.

2

28-31

0

n.a.

11

2

2

30-33

0

n.a.

2

31-34

0

n.a.

12

2

2

30-31

0

n.a.

2

31-32

0

n.a.

  1. Virus isolation was attempted on all PCR-positive samples.
  2. n.a., not applicable.