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Table 2 Virus detection in pharyngeal and cloacal swabs of H5N1-inoculated gulls during the course of the experiment

From: Experimental infection of highly pathogenic avian influenza virus H5N1 in black-headed gulls (Chroicocephalus ridibundus)

   Pharyngeal swab Cloacal swab
   RT-PCR Virus isolation RT-PCR Virus isolation
Day No. tested No. positive Ct range No. positive* Titre range No. positive Ct range No. positive* Titre range
1 16 16 19-27 16 1.5-5.5 6 27-32 1 < 0.8-0.8
2 16 16 21-29 15 1.2-3.5 16 22-30 4 1.5-2.8
3 12 11 21-29 10 0.8-4.2 6 28-31 1 < 0.8-0.8
4 12 12 22-30 9 1.8-3.8 7 26-33 2 0.8-2.2
5 7 7 23-32 6 1.8-3.8 6 24-31 3 1.2-2.2
6 5 5 20-34 4 0.8-3.5 5 20-30 2 2.2-2.5
7 3 3 23-28 3 1.5-3.2 3 27-32 0 n.a.
9 2 2 31 0 n.a. 2 28-31 0 n.a.
11 2 2 30-33 0 n.a. 2 31-34 0 n.a.
12 2 2 30-31 0 n.a. 2 31-32 0 n.a.
  1. Virus isolation was attempted on all PCR-positive samples.
  2. n.a., not applicable.
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Veterinary Research

ISSN: 1297-9716