S . Gallinarum's invasion defect is not related to a T3SS-1 gene-expression defect. RNA from LA5 and 287/91 strains were collected after 0.5 h of contact with CLEC213 and were reverse transcribed. sipA, hilA and invF cDNA was amplified using PCR after DNase treatment and reverse transcription (+) or not (-). Housekeeping gene tufA expression was measured as a control for RNA quantity. Band intensity was quantified using Quantity One software (BioRad). The number above each band represents the ratio of SG287/91 divided by SE LA5 intensities for each gene. Three independent experiments were performed.