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Figure 3 | Veterinary Research

Figure 3

From: Deciphering why Salmonella Gallinarum is less invasive in vitro than Salmonella Enteritidis

Figure 3

S. Gallinarum and S. Enteritidis mainly invade cells through a T3SS-1-dependent pathway but S. Gallinarum invasive ability is delayed compared to S. Enteritidis. A. T3SS-1 inactivated mutants of S. Gallinarum and S. Enteritidis show the same relative entry defect. Gentamicin protection assays were performed in avian (LMH, CLEC213, DF-1) and human (HeLa) cell lines. Infection was carried out for 0.5 h with wild-type strains (black bars) and their invA mutants (grey bars). Results obtained for the invA mutants are expressed relative to values obtained for the wild-type strains, arbitrarily set at 100%. Raw data are presented in Additional file 2. Values represent means× μstandard deviation of two independent experiments performed in duplicate. B. S. Gallinarum's T3SS-1 dependent invasive ability is delayed in avian cell lines compared to S. Enteritidis. Gentamicin protection assays were performed in avian (LMH, CLEC213, DF-1) and human (HeLa) cell lines. Infection was carried out for 0.5 h to 4.5 h using SE LA5, SG 287/91 strains and their respective invA mutants. To assess the role of the T3SS-1, the number of intracellular bacteria recovered for the mutant strain was subtracted from the number recovered from the wild-type strain at each time point. The grey line represents T3SS-1-dependent entry of the SE LA5 strain and the black line represents that of the strain SG 287/91. The results correspond to the mean× μstandard deviation of two independent experiments performed in duplicate and are expressed in log10 CFU mL-1.

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