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Table 3 The 27 large-scale purified proteins

From: In silico prediction of Gallibacterium anatis pan-immunogens

Protein ID

Description

Form

P/N ratioa

Acc. no.

GtxA-Nc

RTX toxin (first half part)

Soluble

20.1***

FJ917356

Gab_0523

Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Soluble

7.9***

ERF78926.1

Gab_2156d

F17-like fimbrial subunit

Soluble

6.6***

ERF79559.1

Gab_0091

Outer membrane lipoprotein

Insoluble

5.9***

ERF79624.1

Gab_2348

Outer membrane protein

Insoluble

4.1***

ERF79081.1

FlfAd

F17-like fimbrial subunit

Soluble

2.5***

JX855927

Gab_1755

Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Insoluble

1.8***

ERF79542.1

Gab_1309

Membrane proteins related to metalloendopeptidases

Soluble

1.7***b

ERF77527.1

Gab_2312

Autotransporter adhesion

Soluble

1.7***b

ERF77433.1

GtxA-Cc

RTX toxin (last half part)

Insoluble

1.4***

FJ917356

Gab_1576

Outer membrane receptor for ferrienterochelin and colicins

Insoluble

1.6*

ERF78509.1

Gab_1654

Outer membrane phospholipase A

Insoluble

1.3*

ERF79322.1

Gab_2224

Outer membrane receptor proteins, mostly Fe transport

Insoluble

1.3

ERF78217.1

Gab_2192

Outer membrane protein W

Insoluble

1.2

ERF78317.1

Gab_1450

Opacity protein and related surface antigens

Insoluble

1.2

ERF79004.1

Gab_0652

Organic solvent tolerance protein OstA

Insoluble

1.2

ERF78644.1

Gab_1283

Long-chain fatty acid transport protein

Insoluble

1.2

ERF77302.1

Gab_0047

Metal-dependent proteases with possible chaperone activity

Soluble

1.0

ERF77629.1

Gab_1162

P pilus assembly protein, porin PapC

Insoluble

1.0

ERF79423.1

Gab_1192

Surface lipoprotein

Insoluble

1.0

ERF78832.1

Gab_1397

Outer membrane protein

Insoluble

1.0

ERF79124.1

Gab_0925

Hypothetical protein

Insoluble

1.0

ERF77284.1

Gab_0186

Membrane-bound lytic murein transglycosylase

Insoluble

1.0

ERF78366.1

Gab_2304

Glycerophosphoryl diester phosphodiesterase

Soluble

0.9

ERF77421.1

OmpCe

Outer membrane protein (porin)

Insoluble

0.9

KF160335

Gab_0572d

F17-like fimbrial subunit

Insoluble

0.9

ERF79277.1

Gab_1245

Hemolysin activation/secretion protein

Insoluble

0.9

ERF78979.1

  1. aRatio between the mean antibody titer values (P = hyper-immune serum; N = pre-immune serum). Asterisks indicate statistically significant difference between the two groups (* = P < 0.05, *** P < 0.001).
  2. bProteins ranked within the Top20 of the G. anatis 12656–12 proteome by VacFinder®.
  3. cPreviously described in [35]. Due to the size of the protein it was cloned, expressed and purified as two halves.
  4. dPreviously described in [24].
  5. ePreviously described in [36].