Table 2 The 42 proteins from G. anatis 12656–12 selected for cloning and expression
From: In silico prediction of Gallibacterium anatis pan-immunogens
Protein ID | Description | Mw (kDa) | PSORTb prediction | TMHMM prediction | SignalP prediction | Acc. no. |
|---|---|---|---|---|---|---|
Gab_0001 | Hypothetical protein | 195.9 | Outer membrane | 0 | No | ERF78007.1 |
Gab_0047 | Metal-dependent proteases with possible chaperone activity | 36.5 | Extracellular | 0 | No | ERF77629.1 |
Gab_0087 | Hypothetical protein | 140.9 | Extracellular | 0 | No | ERF78219.1 |
Gab_0091 | Outer membrane lipoprotein | 15.3 | Unknown | 0 | Yes | ERF79624.1 |
Gab_0151a | RTX toxins and related Ca2 + −binding proteins | 216.4 | Extracellular | 0 | No | FJ917356 |
Gab_0178 | Predicted secreted acid phosphatase | 30.5 | Unknown | 0 | Yes | ERF78374.1 |
Gab_0186 | Membrane-bound lytic murein transglycosylase | 39.9 | Unknown | 0 | Yes | ERF78366.1 |
Gab_0337 | Autotransporter adhesin | 142.3 | Extracellular | 0 | No | ERF78595.1 |
Gab_0523 | Outer membrane protein and related peptidoglycan-associated (lipo)proteins | 16.4 | Outer membrane | 0 | Yes | ERF78926.1 |
Gab_0572b | F17-like fimbrial subunit | 19.1 | Extracellular | 0 | Yes | ERF79277.1 |
Gab_0574 | P pilus assembly protein, porin PapC | 94.2 | Outer membrane | 0 | Yes | ERF79276.1 |
Gab_0602 | Outer membrane protein | 47.8 | Outer membrane | 0 | Yes | ERF78505.1 |
Gab_0652 | Organic solvent tolerance protein OstA | 90.1 | Outer membrane | 0 | Yes | ERF78644.1 |
Gab_0661 | Small protein A (tmRNA-binding) | 16 | Unknown | 0 | Yes | ERF78651.1 |
Gab_0925 | Hypothetical protein | 85.2 | Outer membrane and/or extracellular | 0 | Yes | ERF77284.1 |
Gab_0999 | Rare lipoprotein B | 18.2 | Unknown | 1 | Yes | ERF79357.1 |
Gab_1008 | Type II secretory pathway, component HofQ | 42.1 | Outer membrane | 0 | No | ERF79423.1 |
Gab_1162 | P pilus assembly protein, porin PapC | 93.8 | Outer membrane | 0 | Yes | ERF78846.1 |
Gab_1164b | F17-like fimbrial subunit | 20.5 | Extracellular | 0 | Yes | JX855927 |
Gab_1192 | Surface lipoprotein | 28 | Cytoplasmic membrane | 0 | Yes | ERF78832.1 |
Gab_1245 | Hemolysin activation/secretion protein | 67.8 | Outer membrane | 1 | Yes | ERF78979.1 |
Gab_1283 | Long-chain fatty acid transport protein | 48.8 | Outer membrane | 0 | Yes | ERF77302.1 |
Gab_1309 | Membrane proteins related to metalloendopeptidases | 42.7 | Unknown | 0 | Yes | ERF77527.1 |
Gab_1396 | Uncharacterized protein conserved in bacteria | 146.6 | Outer membrane | 1 | No | ERF79175.1 |
Gab_1397 | Outer membrane protein | 65.9 | Outer membrane | 0 | Yes | ERF79124.1 |
Gab_1399 | Membrane-bound metallopeptidase | 47.2 | Outer membrane and/or extracellular | 0 | Yes | ERF79126.1 |
Gab_1450 | Opacity protein and related surface antigens | 23.1 | Outer membrane | 1 | Yes | ERF79004.1 |
Gab_1576 | Outer membrane receptor for ferrienterochelin and colicins | 23.4 | Outer membrane | 0 | No | ERF78509.1 |
Gab_1631 | Cell envelope opacity-associated protein A | 43.9 | Extracellular | 1 | No | ERF79417.1 |
Gab_1654 | Outer membrane phospholipase A | 32.4 | Outer membrane | 1 | Yes | ERF79322.1 |
Gab_1755 | Outer membrane protein and related peptidoglycan-associated (lipo)proteins | 27.9 | Outer membrane | 0 | Yes | ERF79542.1 |
Gab_2087 | Outer membrane protein | 51 | Outer membrane | 0 | Yes | ERF78059.1 |
Gab_2124c | Outer membrane protein (porin) | 41.1 | Outer membrane | 0 | Yes | KF160335 |
Gab_2156b | F17-like fimbrial subunit | 20.7 | Extracellular | 0 | Yes | ERF79559.1 |
Gab_2158 | P pilus assembly protein, porin PapC | 90.6 | Outer membrane | 0 | Yes | ERF79560.1 |
Gab_2192 | Outer membrane protein W | 25.7 | Outer membrane | 0 | Yes | ERF78317.1 |
Gab_2224 | Outer membrane receptor proteins, mostly Fe transport | 81 | Outer membrane | 0 | Yes | ERF78217.1 |
Gab_2274 | Outer membrane receptor proteins, mostly Fe transport | 74 | Outer membrane | 0 | Yes | ERF77464.1 |
Gab_2304 | Glycerophosphoryl diester phosphodiesterase | 41.6 | Unknown | 0 | Yes | ERF77421.1 |
Gab_2312 | Autotransporter adhesin | 325.7 | Outer membrane and/or extracellular | 0 | No | ERF77433.1 |
Gab_2347 | Outer membrane protein/protective antigen OMA87 | 89.3 | Outer membrane | 0 | Yes | ERF79042.1 |
Gab_2348 | Outer membrane protein | 19.8 | Outer membrane | 0 | Yes | ERF79081.1 |