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Table 1 Bacterial strains and plasmids used in this work

From: Mutants in the lipopolysaccharide of Brucella ovis are attenuated and protect against B. ovis infection in mice

Bacterial strains/Plasmids Characteristic Source/Reference
Brucella ovis   
PA virulent strain, natural NalR CITA collection
BoΔwadA PA in frame deletion mutant in wadA Δ58-679 This work
BoΔwadB PA in frame deletion mutant in wadB Δ49-195 This work
BoΔwadC PA in frame deletion mutant in wadC Δ17-306 This work
BoPA-Gm challenge strain, PA GmR UN collection
Brucella melitensis   
Rev 1 reference vaccine CITA collection
E. coli   
S17-1 λpir mating strain with plasmid RP4 inserted into the chromosome Simon et al. [[19]]
TOP10 F’ FlacI q Tn 10 (TetR) mcrA Δ(mrr-hsdRMS-mcrBC) 80lacZ ΔM15 ΔlacX74 recA1alaD139 endA1 nupG Invitrogen
Plasmids   
pCR2.1 cloning vector Invitrogen
pJQK derivated of pJQ200KS+; KmR, GmS Scupham and Triplett [[20]]
pYRI-12 913-bp of B. abortus parental chromosomal DNA containing the wadA deletion allele, generated by PCR and cloned into pCR2.1 This work
pYRI-13 BamHI-XbaI fragment from pYRI-12 cloned into the corresponding sites of pJQK This work
pYRI-1 570-bp of B. abortus parental chromosomal DNA containing the wadB deletion allele, generated by PCR and cloned into pCR2.1 Gil-Ramírez et al. [[7]]
pYRI-2 BamHI-XbaI fragment from pYRI-1 cloned into the corresponding sites of pJQK Gil-Ramírez et al. [[7]]
pYRI-14 934-bp of B. ovis parental chromosomal DNA containing the wadC deletion allele, generated by PCR and cloned into pCR2.1 This work
pYRI-15 BamHI-XbaI fragment from pYRI-14 cloned into the corresponding sites of pJQK This work
  1. CITA, Centro de investigación y tecnología agroalimentaria; UN, Universidad de Navarra.