H5N1 HPAIV NS1 interferes with (A-C) antiviral molecules, (D-I) proinflammatory cytokines and (J-L) RLR induction. DEF cells were infected with DK212 or rDK212-ΔNS1 at different doses. After 24 h quantitative real-time PCR tests were performed. *The difference (P < 0.05) between the experimental and control group. Error bars indicate standard deviations.