Babesia spp. in European wild ruminant species: parasite diversity and risk factors for infection

Table 2 Primer sequences and PCR conditions used in this study

Primer designation	Specificity	Locus	Sequence (5’-3’)	Fragment size	Annealing temp (°C)	Extension time (s)	No. cycles	Reference
BabsppF1	Babesia spp.*	18S rRNA gene	GTTTCTGMCCCATCAGCTTGAC	422-440	61	45	40	[25]
BabsppR	Babesia spp.*	18S rRNA gene	CAAGACAAAAGTCTGCTTGAAAC
BabcapF	Babesia capreoli	rRNA locus (ITS2)	AGGAACCACACTTTTACTGGTTT	210	62	30	40	This study
BabcapR	Babesia capreoli	rRNA locus (ITS2)	CATCCACTTGCYATAGAAATACAA
BabsppF1	Babesia sp. EU1	18S rRNA gene	GTTTCTGMCCCATCAGCTTGAC	362	61	45	40	[25]
BabEU1	Babesia sp. EU1	18S rRNA gene	AGACAAGAGTCAATAACTCGATAAC

*Bovine Babesia spp.: B. bigemina, B. capreoli, B. canis, B. crassa, B. divergens, B. major, B. motasi, B. odocoilei, B. ovata, Babesia sp. EU1.

ISSN: 1297-9716