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Table 1 Experimental groups: clinical/histopathology analyses and serology

From: The effect of the TLR9 ligand CpG-oligodeoxynucleotide on the protective immune response to alcelaphine herpesvirus-1-mediated malignant catarrhal fever in cattle

Group1 Days after challenge2 Pathology/histology3 Viral DNA4 NS ab5 NS ab Plas ab6 Plas ab
     Pre-chall After chall Pre-chall After chall
Gp 1: Virus + Em        
841 99 No MCF lesions NDet 67 79 82 58
920 99 No MCF lesions NDet 24 89 44 60
221 98 No MCF lesions NDet 63 66 42 44
983 98 No MCF lesions NDet 30 50 132 248
001 91 No MCF lesions NDet 195 118 72 109
371 89 No MCF lesions NDet 151 138 79 220
675 89 No MCF lesions NDet 44 70 89 114
635 63 MCF Lesions: Lymphocytic inflammation and vasculitis. Pneumonia 32.6 35 96 47 449
101 42 Mild MCF lesions: Lymphocytic inflammation and vasculitis. Pneumonia 32.5 55 220 37 421
Gp 2: Virus + Em +CpG        
105 98 No MCF lesions NDet 72 118 89 149
106 98 No MCF lesions NDet 40 66 116 198
706 97 No MCF lesions NDet 85 66 75 73
679 96 No MCF lesions NDet 29 17 81 128
236 91 No MCF lesions NDet 9 24 64 214
162 90 No MCF lesions NDet 64 97 133 218
663* 90 Mild MCF lesions. Lymphocytic inflammation and vasculitis 33.6 6 234 51 931
400678 41 Mild MCF lesions: Lymphocytic inflammation and vasculitis. Mild pneumonia 37.5 0 300 60 324
065 35 MCF Lesions: Lymphocytic inflammation and vasculitis in multiple tissues. Pneumonia 27 0 408 26 299
Gp 3: Virus + CpG        
666 90 No MCF lesions NDet 0 25 46 50
021 89 No MCF lesions NDet 19 19 23 68
169 89 No MCF lesions NDet 50 64 85 139
201027 65 Mild MCF lesions. Lymphocytic inflammation and vasculitis. Pneumonia 27.6 0 40 0 0
273 36 MCF Lesions. Lymphocytic inflammation and vasculitis. Pneumonia 26.2 0 0 0 220
Gp 4: Adjuvant        
658 88 No MCF lesions NDet 0 0 0 0
101027 60 Mild MCF lesions. Lymphocytic inflammation 30.3 0 0 0 0
249 36 Mild MCF lesions. Lymphocytic inflammation and vasculitis in the brain and liver only NDet 0 90 0 104
925 36 MCF Lesions. Lymphocytic inflammation and vasculitis. Pneumonia 24.7 2 74 0 0
986 27 MCF lesions: Lymphocytic vasculitis in multiple tissues. Pneumonia 30 0 15 0 81
  1. 1Em = emulsigen adjuvant, CpG = oligoCpG adjuvant.
  2. 2Time from challenge to euthanasia/post-mortem examination.
  3. 3Tissues in this analysis were: brain, lung, liver, kidney, prescapular lymph node, alimentary tract epithelium and mesenteric lymph node.
  4. 4Viral DNA was assayed in terminal blood samples by qPCR to give a specific and internally-controlled diagnostic test. Data are expressed as Ct values – the cycle number at which specific amplification was first detected. Moderate/severe MCF usually has values in the range 21–29. NDet = specific amplification of AlHV-1 DNA not detected (but control actin-specific PCR was detected). *This animal had histological evidence of infection.
  5. 5Nasal secretion antibody (NS ab) titres pre-challenge and after challenge with virus.
  6. 6Plasma antibody (Plas ab) titres pre-challenge and after challenge with virus.
  7. Animals in groups 1, 2 and 3 received the same dose of vaccine (1 mL of 107 TCID50 per mL) for both prime and boost, given intramuscularly and in groups 1 and 2 contained 20%(v/v) Emulsigen. Control animals in group 4 received virus-free culture medium with Emulsigen prepared and administered in the same way. Animals in all groups were challenged 23 weeks after boost with 10 mL of 103.8 TCID50/mL of virulent AlHV-1 given intranasally. For mild MCF lesions, Viral DNA in the tissues can be below detection level.