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Table 2 Cytopathic effect (CPE) observed in E-11 cell monolayers infected with RGNNV, SJNNV, RGNNV/SJNNV, SJNNV/RGNNV betanodavirus strains at different incubation temperatures

From: In vitro study of the replication capacity of the RGNNV and the SJNNV betanodavirus genotypes and their natural reassortants in response to temperature

  15 °C 20 °C 25 °C 30 °C
  RGNNV SJNNV RGNNV/SJNNV SJNNV/RGNNV RGNNV SJNNV RGNNV/SJNNV SJNNV/RGNNV RGNNV SJNNV RGNNV/SJNNV SJNNV/RGNNV RGNNV SJNNV RGNNV/SJNNV SJNNV/RGNNV
T1 § § § § - - - - - - - - - - - -
T3 § § § § - - - - - - - - + + + - + + + -
T5 § § § § + + + + + + + + + + + + + + + + - + + + + +
T7 * * * * + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
T8 * * * * + + + + + + + + + + + + + + + + + + + +       + + +   + + +
T9 * * * *           + + + +   + + + +
T10 * * * *           + + + +   + + + +
  1. Data were recorded at T1 (20 hpi), T3 (45 hpi), T5 (69 hpi), T7 (93 hpi), T8 (117 hpi), T9 (141 hpi) and T10 (165 hpi). Cells appearance, estimation of the CPE and its degree of severity were assessed according to the following levels: cell shrinkage (§); alteration of the cell monolayer characterized by dark and contracted cells with the tendency to detach from the surface of the flask (*); absence of CPE (-); cellular alteration and presence of diffuse vacuoles (+); presence of diffuse foci of rounded, granular and vacuolated cells (+ +); presence of diffuse foci of rounded, granular and vacuolated cells and initial cell monolayer disruption (+ + +); presence of diffuse foci of rounded, granular and vacuolated cells and advanced cell monolayer disruption (+ + + +); complete cell monolayer disruption (+ + + + +).