Evaluation of a 3A-truncated foot-and-mouth disease virus in pigs for its potential as a marker vaccine

Table 2 Responses of swine directly inoculated with r-HN or r-HN/3A _93–143

Virus	Pig^a	Maximum viremia titre^b(dpi)	Fever^c(dpi)	Maximum clinical Score^d(dpi)	Maximum LPBE-antibody^e(dpi)	3ABC antibody^f(dpi)
r-HN	2408	7.23 (2)	Yes (2 and 3)	4/5 (3)	1:1024 (14)	+
r-HN	2411	7.7 (2)	Yes (2 to 5)	3/5 (4)	1:512 (14)	+
r-HN	2412	6.9 (3)	Yes (3 to 5)	4/5 (5)	1:720 (7)	+
r-HN/3A_93–143	2413	7.1 (2)	Yes (3 to 5)	4/5 (4)	1:720 (14)	+
r-HN/3A_93–143	2421	6.0 (3)	Yes (3 to 5)	2/5 (3)	1:512 (14)	+
r-HN/3A_93–143	2422	7.3 (3)	Yes (4 to 5)	2/5 (5)	1:720 (7)	+

^aPigs were inoculated with 10⁶ TCID₅₀ of r-HN or r-HN/3A_93–143 by intradermal route.
^bValues are expressed as the log₁₀ RNA copies/mL. Dpi here indicates the day(s) post-inoculation that the virus peak was detected.
^cFever defined as rectal temperature > 40 °C. Dpi here indicates the days when fever was detected.
^dClinical scores were scored as previously described [24]. The maximum score is 5. Dpi here indicates the day after inoculation that the maximum score was reached.
^eLPBE-antibody, liquid-phase blocking ELISA antibody. Dpi here indicates the day after inoculation that the maximum LPBE-antibodies were reached.
^f3ABC antibody, the antibody to the nonstructural protein 3ABC of FMDV. Dpi here indicates four-weeks after inoculation that 3ABC antibodies were detected. +, positive, −, negative.

ISSN: 1297-9716