Evaluation of a 3A-truncated foot-and-mouth disease virus in pigs for its potential as a marker vaccine

Table 1 Primers used for the construction of the mutant FMDV full-length cDNA clone with the targeted deletion in NSP 3A

Primer	Sequence(5′ → 3′)	Usage
HN-1 F	CAAGAAGTGATTGAGCGGGT	Amplification of fragment A
3A93-143R	GGTTGTTCCCTCCCGGGCACGTCATCCAGTGAGTCATCCA	Amplification of fragment A
3A93-143 F	TGGATGACTCACTGGATGACGTGCCCGGGAGGGAACAACC	Amplification of fragment B
HN-4R	GTTCCCTTCTTCATTCTCGC	Amplification of fragment B

ISSN: 1297-9716