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Table 1 List of bovine immunological reagents

From: Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle

Restricting BoLA class I molecule

CTL Lines

T. parva antigen

CTL Epitope

1) 6*01301

BB007 & BV115

Tp1

214VGYPKVKEEML224

2) 6*04101

BW02 (Clones 5 & 7)

Tp2

27SHEELKKLGML37

3) 2*01201

592

Tp2

49KSSHGMGKVGK59

4) BoLA-T2c

Bx196

Tp2

96FAQSLVCVL104

5) BoLA-T5

BV50

Tp5

87SKADVIAKY95

6) 3*00101

Bx63

Tp8

379CGAELNHFL387

7) 1*02301

495

Tp9

67AKFPGMKKSK76

  1. The Table lists the BoLA class I molecules that were expressed in this study, the panel of CTL lines used to test p-MHC class I tetramers and their antigen and epitope specificity. The bulk CTL lines were prepared from cattle immunized with a live parasite vaccine as previously described [29]. The peptide specificity of each CTL line was confirmed by ELISpot assay (data not shown).