Restricting BoLA class I molecule
|
CTL Lines
|
T. parva antigen
|
CTL Epitope
|
---|
1) 6*01301
|
BB007 & BV115
|
Tp1
|
214VGYPKVKEEML224
|
2) 6*04101
|
BW02 (Clones 5 & 7)
|
Tp2
|
27SHEELKKLGML37
|
3) 2*01201
|
592
|
Tp2
|
49KSSHGMGKVGK59
|
4) BoLA-T2c
|
Bx196
|
Tp2
|
96FAQSLVCVL104
|
5) BoLA-T5
|
BV50
|
Tp5
|
87SKADVIAKY95
|
6) 3*00101
|
Bx63
|
Tp8
|
379CGAELNHFL387
|
7) 1*02301
|
495
|
Tp9
|
67AKFPGMKKSK76
|
- The Table lists the BoLA class I molecules that were expressed in this study, the panel of CTL lines used to test p-MHC class I tetramers and their antigen and epitope specificity. The bulk CTL lines were prepared from cattle immunized with a live parasite vaccine as previously described [29]. The peptide specificity of each CTL line was confirmed by ELISpot assay (data not shown).