Use of “one-pot, mix-and-read” peptide-MHC class I tetramers and predictive algorithms to improve detection of cytotoxic T lymphocyte responses in cattle

Table 1 List of bovine immunological reagents

Restricting BoLA class I molecule	CTL Lines	*T. parva* antigen	CTL Epitope
1) 6*01301	BB007 & BV115	Tp1	²¹⁴VGYPKVKEEML₂₂₄
2) 6*04101	BW02 (Clones 5 & 7)	Tp2	²⁷SHEELKKLGML₃₇
3) 2*01201	592	Tp2	⁴⁹KSSHGMGKVGK₅₉
4) BoLA-T2c	Bx196	Tp2	⁹⁶FAQSLVCVL₁₀₄
5) BoLA-T5	BV50	Tp5	⁸⁷SKADVIAKY₉₅
6) 3*00101	Bx63	Tp8	³⁷⁹CGAELNHFL₃₈₇
7) 1*02301	495	Tp9	⁶⁷AKFPGMKKSK₇₆

The Table lists the BoLA class I molecules that were expressed in this study, the panel of CTL lines used to test p-MHC class I tetramers and their antigen and epitope specificity. The bulk CTL lines were prepared from cattle immunized with a live parasite vaccine as previously described [29]. The peptide specificity of each CTL line was confirmed by ELISpot assay (data not shown).

ISSN: 1297-9716