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Table 2 Ct values of FCoV RNA extracted from clinical samples and the amino acids coded at position 1058 and position 1060 in the FCoV S protein

From: Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis

Group

Cat number

Type of sample

Ct value

Codon at position 1058

Non FIP

33

Tissue

35.8a

UUG (Leu)

   

Noneb

Not applicable

   

Nonec

Not applicable

Non FIP

38

Faeces

34.1

AUG (Met)

  

Tissue

Noned

Not applicable

   

Nonee

Not applicable

Non FIP

41

Faeces

26.8

AUG (Met)

  

Tissue

Noneb

Not applicable

   

Nonef

Not applicable

Non FIP

48

Faeces

34.2

AUG (Met)

  

Tissue

35.7b

UUG (Leu)

   

35.8a

UUG (Leu)

Non FIP

51

Tissue

37.3f

UUG (Leu)

Non FIP

52

Faeces

None

Not applicable

  

Tissue

Noneg

Not applicable

Non FIP

54

Faeces

None

Not applicable

  

Tissue

37.3h

UUG (Leu)

   

Nonei

Not applicable

   

Noneb

Not applicable

   

Nonej

Not applicable

   

Nonec

Not applicable

   

Noned

Not applicable

Non FIP

56

Tissue

35.9b

UUG (Leu)

   

Noned

Not applicable

   

Nonec

Not applicable

   

28.6k

AUG (Met)1

Non FIP

57

Faeces

None

Not applicable

  

Tissue

31.6l

UUG (Leu)

   

33.4b

UUG (Leu)

   

Nonec

Not applicable

Non FIP

59

Faeces

33.6

AUG (Met)

  

Tissue

Noneb

Not applicable

Non FIP

60

Faeces

35.6

AUG (Met)/CUG (Leu)

  

Tissue

Noneb

Not applicable

Non FIP

63

Tissue

Nonem

Not applicable

Non FIP

65

Faeces

None

Not applicable

  

Tissue

Nonel

Not applicable

   

Noneb

Not applicable

Non FIP

69

Faeces

26.3

AUG (Met)

  

Tissue

Nonec

Not applicable

   

Noneb

Not applicable

   

Nonel

Not applicable

   

Noned

Not applicable

Non FIP

71

Tissue

Noneb

Not applicable

   

Nonea

Not applicable

   

Nonej

Not applicable

Non FIP

72

Tissue

Noneb

Not applicable

   

Nonej

Not applicable

   

Nonel

Not applicable

   

Noned

Not applicable

   

Nonec

Not applicable

FIP

26

Tissue

17.1n

UUG (Leu)

FIP

27

Tissue

26.2b

UUG (Leu)

FIP

28

Faeces

19.3

AUG (Met)

  

Tissue

25.5b

UUG (Leu)

   

26.9j

UUG (Leu)

FIP

30

Tissue

29.3b

UUG (Leu)

FIP

31

Faeces

22.7

AUG (Met)

  

Tissue

22.2f

UUG (Leu)

FIP

32

Faeces

21.0

AUG (Met)

  

Tissue

17.6a

UUG (Leu)

   

19.2l

UUG (Leu)

   

26.2b

UUG (Leu)

FIP

34

Tissue

20.4l

UUG (Leu)

FIP

35

Tissue

20.5b

UUG (Leu)

FIP

36

Tissue

31.1l

UUG (Leu)

FIP

37

Faeces

15.8

AUG (Met)

  

Tissue

16.2o

AUG (Met)2

FIP

42

Faeces

19.9

AUG (Met)

  

Tissue

20.4l

UUG (Leu)

   

21.6b

UUG (Leu)

FIP

43

Tissue

24.1a

CUG (Leu)

FIP

44

Faeces

30.0

AUG (Met)

  

Tissue

19.0p

UUG (Leu)

FIP

45

Tissue

25.0q

UUG (Leu)

   

27.9r

UUG (Leu)

FIP

46

Faeces

34.2

UUG (Leu)

  

Tissue

31.7l

UUG (Leu)

FIP

47

Faeces

33.3

UUG (Leu)

  

Tissue

19.4s

UUG (Leu)

FIP

49

Faeces

30.9

AUG (Met)

  

Tissue

21.8c

UUG (Leu)

FIP

50

Faeces

None

Not applicable

  

Tissue

16.1c

UUG (Leu)

FIP

53

Faeces

21.9

AUG (Met)

  

Tissue

15.5o

UUG (Leu)

FIP

55

Tissue

17.7c

CUG (Leu)

   

20.2b

CUG (Leu)

FIP

58

Faeces

None

Not applicable

  

Tissue

Noneb

Not applicable

FIP

61

Faeces

23.1

AUG (Met)

  

Tissue

Noneb

Not applicable

FIP

62

Tissue

15.3b

UUG (Leu)

   

17.8j

UUG (Leu)

   

18.0a

UUG (Leu)

   

18.9d

UUG (Leu)

   

20.7l

UUG (Leu)

   

30.9c

UUG (Leu)

FIP

66

Faeces

25.5

UUG (Leu)

  

Tissue

16.7j

UUG (Leu)

   

24.7b

UUG (Leu)

FIP

67

Faeces

31.6

AUG (Met)

FIP

68

Tissue

31.1t

UUG (Leu)

   

31.3l

UUG (Leu)

   

33.1b

UUG (Leu)

   

34.0j

UUG (Leu)

   

36.9d

UUG (Leu)

FIP

70

Faeces

None

Not applicable

  

Tissue

23.3b

AUG (Met)1

   

29.3d

AUG (Met)1

   

30.3l

AUG (Met)1

   

31.0j

UUG (Leu)

  1. Tissue types are defined by superscripts: aomentum, bliver, ckidney, dlung, estomach, flymph node, gcerebrum, habdominal lymph node, iheart, jspleen, kcolon, lmesenteric lymph node, mbrain, nmesentery, opleura, ppericardium, qmedulla, rpons, ssmall intestine, tintestine. The amino acid coded at position 1060 in the S protein of FCoV RNA from tissue samples without the M1058L substitution is defined by the superscripts: 1UCU (Ser) and 2GCU (Ala).