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Table 2 Ct values of FCoV RNA extracted from clinical samples and the amino acids coded at position 1058 and position 1060 in the FCoV S protein

From: Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis

Group Cat number Type of sample Ct value Codon at position 1058
Non FIP 33 Tissue 35.8a UUG (Leu)
    Noneb Not applicable
    Nonec Not applicable
Non FIP 38 Faeces 34.1 AUG (Met)
   Tissue Noned Not applicable
    Nonee Not applicable
Non FIP 41 Faeces 26.8 AUG (Met)
   Tissue Noneb Not applicable
    Nonef Not applicable
Non FIP 48 Faeces 34.2 AUG (Met)
   Tissue 35.7b UUG (Leu)
    35.8a UUG (Leu)
Non FIP 51 Tissue 37.3f UUG (Leu)
Non FIP 52 Faeces None Not applicable
   Tissue Noneg Not applicable
Non FIP 54 Faeces None Not applicable
   Tissue 37.3h UUG (Leu)
    Nonei Not applicable
    Noneb Not applicable
    Nonej Not applicable
    Nonec Not applicable
    Noned Not applicable
Non FIP 56 Tissue 35.9b UUG (Leu)
    Noned Not applicable
    Nonec Not applicable
    28.6k AUG (Met)1
Non FIP 57 Faeces None Not applicable
   Tissue 31.6l UUG (Leu)
    33.4b UUG (Leu)
    Nonec Not applicable
Non FIP 59 Faeces 33.6 AUG (Met)
   Tissue Noneb Not applicable
Non FIP 60 Faeces 35.6 AUG (Met)/CUG (Leu)
   Tissue Noneb Not applicable
Non FIP 63 Tissue Nonem Not applicable
Non FIP 65 Faeces None Not applicable
   Tissue Nonel Not applicable
    Noneb Not applicable
Non FIP 69 Faeces 26.3 AUG (Met)
   Tissue Nonec Not applicable
    Noneb Not applicable
    Nonel Not applicable
    Noned Not applicable
Non FIP 71 Tissue Noneb Not applicable
    Nonea Not applicable
    Nonej Not applicable
Non FIP 72 Tissue Noneb Not applicable
    Nonej Not applicable
    Nonel Not applicable
    Noned Not applicable
    Nonec Not applicable
FIP 26 Tissue 17.1n UUG (Leu)
FIP 27 Tissue 26.2b UUG (Leu)
FIP 28 Faeces 19.3 AUG (Met)
   Tissue 25.5b UUG (Leu)
    26.9j UUG (Leu)
FIP 30 Tissue 29.3b UUG (Leu)
FIP 31 Faeces 22.7 AUG (Met)
   Tissue 22.2f UUG (Leu)
FIP 32 Faeces 21.0 AUG (Met)
   Tissue 17.6a UUG (Leu)
    19.2l UUG (Leu)
    26.2b UUG (Leu)
FIP 34 Tissue 20.4l UUG (Leu)
FIP 35 Tissue 20.5b UUG (Leu)
FIP 36 Tissue 31.1l UUG (Leu)
FIP 37 Faeces 15.8 AUG (Met)
   Tissue 16.2o AUG (Met)2
FIP 42 Faeces 19.9 AUG (Met)
   Tissue 20.4l UUG (Leu)
    21.6b UUG (Leu)
FIP 43 Tissue 24.1a CUG (Leu)
FIP 44 Faeces 30.0 AUG (Met)
   Tissue 19.0p UUG (Leu)
FIP 45 Tissue 25.0q UUG (Leu)
    27.9r UUG (Leu)
FIP 46 Faeces 34.2 UUG (Leu)
   Tissue 31.7l UUG (Leu)
FIP 47 Faeces 33.3 UUG (Leu)
   Tissue 19.4s UUG (Leu)
FIP 49 Faeces 30.9 AUG (Met)
   Tissue 21.8c UUG (Leu)
FIP 50 Faeces None Not applicable
   Tissue 16.1c UUG (Leu)
FIP 53 Faeces 21.9 AUG (Met)
   Tissue 15.5o UUG (Leu)
FIP 55 Tissue 17.7c CUG (Leu)
    20.2b CUG (Leu)
FIP 58 Faeces None Not applicable
   Tissue Noneb Not applicable
FIP 61 Faeces 23.1 AUG (Met)
   Tissue Noneb Not applicable
FIP 62 Tissue 15.3b UUG (Leu)
    17.8j UUG (Leu)
    18.0a UUG (Leu)
    18.9d UUG (Leu)
    20.7l UUG (Leu)
    30.9c UUG (Leu)
FIP 66 Faeces 25.5 UUG (Leu)
   Tissue 16.7j UUG (Leu)
    24.7b UUG (Leu)
FIP 67 Faeces 31.6 AUG (Met)
FIP 68 Tissue 31.1t UUG (Leu)
    31.3l UUG (Leu)
    33.1b UUG (Leu)
    34.0j UUG (Leu)
    36.9d UUG (Leu)
FIP 70 Faeces None Not applicable
   Tissue 23.3b AUG (Met)1
    29.3d AUG (Met)1
    30.3l AUG (Met)1
    31.0j UUG (Leu)
  1. Tissue types are defined by superscripts: aomentum, bliver, ckidney, dlung, estomach, flymph node, gcerebrum, habdominal lymph node, iheart, jspleen, kcolon, lmesenteric lymph node, mbrain, nmesentery, opleura, ppericardium, qmedulla, rpons, ssmall intestine, tintestine. The amino acid coded at position 1060 in the S protein of FCoV RNA from tissue samples without the M1058L substitution is defined by the superscripts: 1UCU (Ser) and 2GCU (Ala).