Figure 8

Relative expression of SOCS1 transcripts in infected NPTr cells in presence of chemical inhibitors. The relative expression of SOCS1 transcripts was measured in newborn porcine trachea (NPTr) cells infected with H3N2 SIV in presence of chemical inhibitors UO126 (ERK1/2), SB202190 (p38), and JAK Inhibitor I (JAK/STAT) at different time points (1 h, 3 h, 8 h, and 24 h). The control groups were cultured either in the presence of 0.1% DMSO (C, green) or in the presence of inhibitor (I, blue). Infected cells were either untreated (V, red) or inhibitor-treated (I + V, orange). Comparisons were made using one way ANOVA test and Tukey’s post-test (n = 5, mean ± SEM). Differences were considered significant when P < 0.05 (*), P < 0.01 (**) or P < 0.001 (***).