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Table 1 Viral load of organs in tissue culture infectious dose 50 (TCID50) and genome copies

From: Limited efficacy of West Nile virus vaccines in large falcons (Falco spp.)

Group Bird dpi Brain Spleen Kidney Lung Liver Heart
    TCID50/mg/copies/mg TCID50/mg/copies/mg TCID50/mg/copies/mg TCID50/mg/copies/mg TCID50/mg/copies/mg TCID50/mg/copies/mg
Group 1 F14 21 0/0 0/0 0/0 0/0 0/0 0/0
Inactivated F15 19 0/4.6 0/0.4 0/0.9 0/0 0/0.1 0/0
Boost: 4 wpv F16 20 0/24.8 0/3.8 0/143 0/0 0/0 0/1.8
Challenge: 8 wpv F17 9 18.7/2530 0/3441 0.2/4479 0/6583 0/290 1164/38565819
F18 21 0/4.7 0/0.9 0/223 0/0 0/0.1 0/2.6
Group 2 F37 19 0/0 0/0 0/0 0/0 0/0 0/0
Inactivated F38 20 0/0 0/0 0/0 0/0 0/0.2 0/0
Boost: 3 + 6 wpv F40 19 0/0.6 0/0 0/0 0/0 0/0 0/0
Challenge: 8 wpv F41 20 0/0 0/1.4 0/0 0/0 0/0 0/0
Group 3 F19 20 0/0 0/0 0/0.6 0/0 0/0 0/0
Recombinant F20 19 0/0 0/0 0/0 0/0 0/0 0/0
Boost: 4 wpv F21 21 50.8/4337 0/0 0/141 0/0 0/0 0/0
Challenge: 8 wpv F22 21 0/0 0/0 0/0 0/0 0/0 0/0
F23 20 0/0 0/0 0/0 0/0 0/0 0/0
Group 4 F44 20 0/6 0/0.1 0/0 0/0 0/0 0/0
Recombinant F45 19 0/0 0/0 0/0 0/0 0/0 0/0
Boost: 3 + 6 wpv F47 21 0/0 0/0 0/0 0/0 0/0 0/0
Challenge: 8 wpv F48 19 0/0 0/0 0/0 0/0 0/0 0/0
Group 5 F13 19 0.6/112 0/17.9 0/3.9 0/0.1 0/0.3 0/4.6
Control F24 20 0/32.1 0/0 0/822 0/0 0/0 0/4.7
No vaccination F27 14 8.7/132202 0/42 0/403 0/36.9 0/16.7 37.9/7794
F36 20 0/23.6 0/0 0/2 0/0 0/0 0/0
F42 5 46.9/515 175/318323 76.2/14400 601/5456 94.4/32119 19/6563
F43 20 0.4/38.2 0/11.4 0/1.5 0/0 0/0.1 0/0.1
F51 8 738/2668 10.7/8631 2896/82091 7558/63844 3023/402895 4380/295311
F55 3 0/12.3 3.3/2376 3.9/796 13.2/465 0.9/249 5.4/380
Group 6 F63 21 0/0 0/0 0/0 0/0 0/0 0/0
Env. Control
  1. Abbreviations: copies = genome copies; dpi = days post infection, Env. = environmental, wpv = weeks post vaccination.
  2. Values are given per mg tissue. High TCID50 were found in birds that died after infection, whereas virus was scarce or absent in birds that survived the whole three-week challenge period. Sensitivity of qRT-PCR was generally higher than virus titration in cell culture. Statistical analysis of viral load was performed for brain, spleen, kidney and heart. There is a statistically significant difference to group 5 in brain, kidney and heart of group 2; spleen, kidney and heart of group 3 and in brain, kidney and heart of group 4.