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Figure 1 | Veterinary Research

Figure 1

From: Limited efficacy of West Nile virus vaccines in large falcons (Falco spp.)

Figure 1

Antibodies in falcons detected by ELISA and micro-virus neutralization test (VNT) during vaccination period. Figure 1A shows competition ELISA (ID Screen© WN competition ELISA, IDVet, Grabels, France) responses. Optical density at 450 nm is converted to signal/noise% (S/N%) ratio (S/N% = ODsample/ODnegative control* 100), with values ≤ 40% considered positive, > 40% and ≤ 50% equivocal and > 50% negative. The threshold for positive results is indicated as a solid red line. Figure 1B displays neutralization antibody responses for all groups. Data are presented in a box-and-whisker plot, where the ends of the whiskers represent the minimum and maximum values, respectively. Outliers are represented as points instead of whisker-ends. The box includes 50% of the values of each group and the line in the middle of each group represents the median value of each group. The double immunization with inactivated vaccine (light blue) led to temporary seroconversion whereas the triple vaccination (dark blue) was more efficient. The recombinant vaccine generally induced only a slight seroconversion. With two shots of the recombinant vaccine (light green) low level and temporary seroconversion occurred, while three shots (dark green) provided a better result measureable by ELISA but not by VNT.

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