High PRV load in RBC detected in Experimental Challenge # 2. (A) Detection of PRV by RT-qPCR in Challenge Experiment # 2 presented by mean (and SD) Ct-values for blood, RBC and spleen. The PCR results from the plasma are shown separately on the right, as the RNA input from the cell free plasma is not directly comparable to the other sample material analyzed. (B) Paired analysis of the PRV Ct-value detected in blood compared with spleen from the same fish, presented as mean Ct-value difference at each time point. Data were analyzed using Wilcoxon matched pairs signed rank test. *p < 0.05. (C) Histopathological changes in the ventricle of the heart at 7 weeks post challenge (wpc) (left) consistent with HSMI, including epicarditis (arrowhead) and inflammation of the compactum myocardium layer (arrow). For comparison a ventricle without inflammatory changes (sampled at 0 wpc), is shown to the right.