High PRV load in blood detected in Experimental Challenge # 1. (A) Detection of PRV by RT-qPCR in Challenge Experiment # 1 presented by mean (and SD) Ct-values in blood, heart and skeletal muscle (SM) (n = 6). (B) Paired analysis of the PRV Ct-value detected in blood compared with samples from heart, skeletal muscle (SM), spleen, head kidney (HK) of the same fish. The results are presented as the mean Ct-value difference at each time point. Data were analyzed using Wilcoxon matched pairs signed rank test. *p < 0.05.