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Table 3 Viral genome load in tissues from succumbing and surviving house sparrows

From: Experimental infection of house sparrows (Passer domesticus) with West Nile virus isolates of Euro-Mediterranean and North American origins

Organs

Real-time RT-PCR results

Succumbing HoSp

Surviving HoSp (21 dpi)

NY99

Spain/2007

Italy/2009

NY99

Spain/2007

Italy/2009

Italy/2008

Heart

Positives/total

2/2

2/2

1/1

4/6

0/6

3/7

0/8

Ct (mean)

16.4

26.0

27.1

36.7

n.d.

37.8

n.d.

Ct (range)

15.7-17.1

24.6-27.5

-

35.8-37.9

-

37.5-38.3

-

Liver

Positives/total

2/2

2/2

1/1

5/6

0/6

2/7

3/8

Ct (mean)

14.2

24.5

29.6

34.5

n.d.

36.9

34.3

Ct (range)

13.5-14.8

22.9-26.2

-

31.1-37.8

-

36.4-37.4

33.0-36.1

Brain

Positives/total

2/2

2/2

1/1

3/6

0/6

3/7

3/8

Ct (mean)

18.0

31.9

27.9

32.3

n.d.

36.4

38.0

Ct (range)

17.8-18.3

31.0-32.8

-

31.3-34.2

-

35.0-39.0

36.7-39.6

Kidney

Positives/total

2/2

2/2

1/1

5/6

4/6

4/7

6/8

 

Ct (mean)

14.6

27.9

23.6

29.0

33.1

33.1

33.3

 

Ct (range)

14.2-15.1

27.6-28.3

-

26.2-33.2

31.2-36.9

28.5-37.3

29.4-39.2

Spleen

Positives/total

2/2

2/2

1/1

2/2

4/6

5/7

6/8

 

Ct (mean)

13.1

23.4

23.8

31.7

35.2

35.0

33.0

 

Ct (range)

13.1-13.1

22.1-24.7

-

31.6-31.7

32.7-39.1

32.3-36.8

29.7-39.6

  1. Viral RNA was estimated by real-time RT-PCR in tissues from five different organs of either succumbing or surviving HoSp, sacrificed 21 dpi with WNV as described in the text. Real-time RT-PCR results are expressed as positive (Ct < 40.0) over total samples analysed in each group, and as average viral genome load, expressed as mean Ct for each tissue. The Ct ranges (maximum and minimum values) obtained in each group are also indicated. (n.d.: not detectable).