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Figure 1 | Veterinary Research

Figure 1

From: Ovine and murine T cell epitopes from the non-structural protein 1 (NS1) of bluetongue virus serotype 8 (BTV-8) are shared among viral serotypes

Figure 1

T cells from BTV-8 inoculated animals recognise antigenic determinants shared by BTV serotypes. C57BL/6 mice inoculated were sacrificed and splenocytes were cultured in the presence of inactivated BTV from different serotypes. (A) Proliferative responses to BTV were detected not only to the serotype used for vaccination (BTV-8) but also to serotype 1 and 4. (B) Similarly, splenocytes produced IFN-γ specifically when in presence of BTV serotype 1, 4 and 8. Data presented are representative of 5 mice inoculated with BTV-8. (C) PBLs from BTV-8 infected sheep were cultured for 5 days in the presence of inactivated BTV from different serotypes and tritiated-thymidine was added in the last 16 h of culture. Proliferative response to BTV-4 and BTV-8 but not BTV-1 was detected in the cultures. Data presented are representative of 6 sheep. (D) PBLs from BTV-8 infected sheep were cultured with inactivated BTV from different serotypes for 48 h and IFN-γ was measured by ELISPOT. IFN-γ was produced to BTV-8 and BTV-4 but not BTV-1. Data presented are representative of 3 sheep. Proliferation is presented as the average cpm of 3H-thymidine incorporated in triplicate cultures and IFN-γ ELISPOT assays as average number of spots in triplicate cultures. *denotes p < 0.05 using an unpaired Student t test (control vs BTV).

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