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Table 2 Predicted immunodominant Campylobacter jejuni KC40 antigens identified with HPLC-MS/MS.

From: Passive immunization to reduce Campylobacter jejuni colonization and transmission in broiler chickens

Spot no.

Protein name

Gene

Mascot score

MW (kDa)

pI

1

Flagellar hook protein

flgE

1928

90.1

4.85

2

Flagellin subunit protein A

flaA

4958

59.2

5.7

 

Co-chaperonin GroEL

groEL

3281

58

5.02

 

Flagellin subunit protein B

flaB

1476

59.4

5.66

 

Flagellin subunit protein B

flaB

1048

26.3

5.52

3

Flagellin subunit protein A

flaA

693

58.8

5.53

 

Co-chaperonin GroEL

groEL

493

58

5.02

4

Major outer membrane protein

omp

3071

43.1

4.54

 

Glutamate-1-semialdehyde-2,1-aminomutase

hemL

356

46.5

8.92

 

Glutathionylspermidine synthase family protein

 

164

44.8

4.59

5

Major outer membrane protein

omp

1011

43.1

4.54

6

Translation elongation factor thermo unstable

eF-Tu

2172

43.6

5.11

 

ATP-dependent Clp protease, ATP-binding subunit

clpX

311

46.1

5.21

 

Major outer membrane protein

omp

66

36

4.53

7

Chemotaxis protein V

cheV

1552

35.8

4.92

 

Translation elongation factor thermo unstable

eF-Tu

461

45

5.43

 

Glycyl-tRNA synthetase alpha subunit

glyQ

148

24.3

4.6

 

Phosphoribosylformylglycinamidine cyclo-ligase

purM

70

36

5.07

8

FKBP-type peptidyl-prolyl cis-trans isomerase

slyD

743

20.5

4.75

 

Chemotaxis protein V

cheV

330

35.8

4.92

 

Inorganic pyrophosphatase

ppa

308

19.4

4.79

 

Translation elongation factor thermo unstable

eF-Tu

226

43.6

5.11

 

Heat shock protein GrpE

grpE

168

20.2

4.79

9

Translation elongation factor thermo unstable

eF-Tu

96

43.6

5.11

10

Branched-chain amino acid aminotransferase

ilvE

551

33.1

6.12

 

Enoyl-(acyl-carrier-protein) reductase

fabI

354

30

5.67

 

Putative methyltransferase

 

174

29.8

5.92

11

Methyl-accepting chemotaxis protein

 

811

40.6

5.5

 

Succinyl-CoA synthetase beta chain

 

502

41.9

5.61

 

Cysteine desulfurase, putative

 

472

43.2

5.83

 

Phosphoglycerate kinase

pgk

216

43.9

6.07

12

Conserved hypothetical protein

 

568

53

6.28

 

Pyruvate kinase

pyk

420

54

5.68

 

Glutamyl-tRNA synthetase

gltX-1

306

53.3

6.19

 

ATP synthase F1, alpha subunit

atpA

343

53.4

5.73

 

Trigger factor

tig

255

50.9

5.69

 

Aspartate ammonia-lyase

aspA

219

52.1

5.5

 

Fumarate hydratase

fumC

131

23.2

6.05

13

ATP synthase F1, alpha subunit

atpA

856

53.4

5.73

 

GMP synthase (glutamine-hydrolyzing)

guaA

580

57.4

6.19

14

ATP synthase F1, alpha subunit

atpA

1014

53.4

5.73

 

Peptide transport system substrate-binding protein

 

488

58.7

6.52

 

Acetyl-CoA carboxylase, biotin carboxylase

accC-2

128

54.9

6.4

15

Putative secreted carboxyl-terminal protease

ctpA

1607

48.3

8.7

16

Putative secreted carboxyl-terminal protease

ctpA

717

48.3

8.7

17

High affinity branched-chain amino acid ABC transporter, periplasmic amino acid-binding protein

 

420

39.9

8.94

 

Hydrogenase expression/formation protein

hypD

151

40.9

8.59

18

Dihydrodipicolinate synthase

dapA

315

33

6.02

 

Putative UDP-glucose 4-epimerase

 

214

35.6

7

 

O-acetylserine sulfhydrylase B

cysM

201

32.5

6.62