Effect of virus-vectored ovine IL-2 and/or GMCSF on CD4 and CD8 T-cells and CD14+monocyte/macrophages in vaccinated goats, following PPRV challenge. Goats were vaccinated and challenged as described in Figure 4. Blood was stained with anti-CD4, anti-CD8 and anti-CD14 monoclonal antibodies directly conjugated to fluorochromes. Cells were also stained for intracellular virus using fluorochrome-labelled anti-PPRV H, C77, monoclonal antibody. Results are expressed as the mean percentage ± SD of CD4+ lymphocytes (A), CD8+ lymphocytes (B) and CD14+ cells (C) in peripheral blood. The percentage of CD4+(D) and CD8+(E) lymphocytes with intracellular staining with C77. Data is presented as the mean percentage ± S.E., 0, 4 and 7 days after PPRV challenge.