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Table 2 Summary of the differentially expressed proteins

From: Edwardsiella tarda Hfq: impact on host infection and global protein expression

Spot no.a NCBI no. Protein name Abrr. MASCOT scoreb Theoretical pI/Mr (kD) Coverage (%)c Fold ratio wild type/wild type (mean ± SD)d
Translation
1e gi|269139520 rpsA gene product [Edwardsiella tarda EIB202]: 30S ribosomal protein S1 RpsA 351 4.61/61.17 41.65 0.42 ± 0.05
3 gi|18858425 tuf2 gene product [Edwardsiella tarda EIB202]: elongation factor Tu Tuf2 381 4.91/43.43 54.31 0.35 ± 0.09
6 gi|269138094 tsf gene product [Edwardsiella tarda EIB202]: translation elongation factor Ts Tsf 223 5.3/30.70 36.84 0.45 ± 0.07
Metabolism
4 gi|294637737 Putative carbamoyltransferase YgeW [Edwardsiella tarda ATCC 23685] YgeW 65.1 6.07/44.38 17.97 0.21 ± 0.12
7 gi|269138485 Dyp-type peroxidase family protein [Edwardsiella tarda EIB202]   260 5.76/33.27 38.46 3.62 ± 0.78
8 gi|269140209 ybaS gene product [Edwardsiella tarda EIB202]: glutaminase YbaS 362 4.83/33.06 81.35 -∞
14 gi|387866356 Putative iron-containing alcohol dehydrogenase [Edwardsiella tarda FL6-60]   218 5.73/42.61 74 0.27 ± 0.06
15 gi|469762796 Cysteine synthase [Edwardsiella tarda C07-087]   199 6.03/33.99 77 0.46 ± 0.05
Transport
5 gi|387867244 Mrp (Multiple resistance and pH adaptation) protein [Edwardsiella tarda FL6-60] Mrp 340 6.22/39.70 26.76 2.81 ± 1.11
Chaperones
10 gi|269140581 fkpA gene product [Edwardsiella tarda EIB202]: FKBP-type peptidyl-prolyl cis-trans isomerase FkpA 425 8.7/28.76 58.39 4.24 ± 1.35
Membrane proteins
11 gi|269140620| ompR gene product [Edwardsiella tarda EIB202]: osmolarity response regulator OmpR 127 6.31/27.41 38.91 -∞
13 gi|304557842 Uncharacterized membrane protein YqjD [Edwardsiella tarda FL6-60] YqjD 600 6.26/10.94 82.18 0.25 ± 0.04
Cellular processes
12 gi|269140157 iscU gene product [Edwardsiella tarda EIB202]: FeS cluster assembly scaffold IscU 102 4.74/13.86 55.47 2.23 ± 0.62
Unknown function and hypothetical protein
2 gi|269139286 Hypothetical protein 1 [Edwardsiella tarda EIB202]   153 5.64/58.02 29.04 -∞
9 gi|269140100 Hypothetical protein 2 [Edwardsiella tarda EIB202]   318 5.33/33.70 54.90 3.21 ± 1.28
16e NA NA NA NA NA NA 5.21 ± 1.25
17 NA NA NA NA NA NA 0.31 ± 0.05
18 NA NA NA NA NA NA 2.94 ± 0.85
19 NA NA NA NA NA NA 4.22 ± 1.77
20 NA NA NA NA NA NA 0.32 ± 0.06
  1. aSpot ID represents the number on the 2-DE gels (Figure 5).
  2. bMOWSE score is -10 log (p), where p is the probability that the observed match is a random event. Based on the NCBInr database using the MASCOT searching program as MS/MS data. Scores greater than 65 are significant (p < 0.05).
  3. cNumber of amino acids spanned by the assigned peptides divided by the protein sequence length.
  4. dMean, the average protein abundance ratio for three paired samples. SD means the standard deviation of protein abundance ratios of one certain spot of three paired samples.
  5. eNot analyzed.