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Table 2 Summary of the differentially expressed proteins

From: Edwardsiella tarda Hfq: impact on host infection and global protein expression

Spot no.a

NCBI no.

Protein name

Abrr.

MASCOT scoreb

Theoretical pI/Mr (kD)

Coverage (%)c

Fold ratio wild type/wild type (mean ± SD)d

Translation

1e

gi|269139520

rpsA gene product [Edwardsiella tarda EIB202]: 30S ribosomal protein S1

RpsA

351

4.61/61.17

41.65

0.42 ± 0.05

3

gi|18858425

tuf2 gene product [Edwardsiella tarda EIB202]: elongation factor Tu

Tuf2

381

4.91/43.43

54.31

0.35 ± 0.09

6

gi|269138094

tsf gene product [Edwardsiella tarda EIB202]: translation elongation factor Ts

Tsf

223

5.3/30.70

36.84

0.45 ± 0.07

Metabolism

4

gi|294637737

Putative carbamoyltransferase YgeW [Edwardsiella tarda ATCC 23685]

YgeW

65.1

6.07/44.38

17.97

0.21 ± 0.12

7

gi|269138485

Dyp-type peroxidase family protein [Edwardsiella tarda EIB202]

 

260

5.76/33.27

38.46

3.62 ± 0.78

8

gi|269140209

ybaS gene product [Edwardsiella tarda EIB202]: glutaminase

YbaS

362

4.83/33.06

81.35

-∞

14

gi|387866356

Putative iron-containing alcohol dehydrogenase [Edwardsiella tarda FL6-60]

 

218

5.73/42.61

74

0.27 ± 0.06

15

gi|469762796

Cysteine synthase [Edwardsiella tarda C07-087]

 

199

6.03/33.99

77

0.46 ± 0.05

Transport

5

gi|387867244

Mrp (Multiple resistance and pH adaptation) protein [Edwardsiella tarda FL6-60]

Mrp

340

6.22/39.70

26.76

2.81 ± 1.11

Chaperones

10

gi|269140581

fkpA gene product [Edwardsiella tarda EIB202]: FKBP-type peptidyl-prolyl cis-trans isomerase

FkpA

425

8.7/28.76

58.39

4.24 ± 1.35

Membrane proteins

11

gi|269140620|

ompR gene product [Edwardsiella tarda EIB202]: osmolarity response regulator

OmpR

127

6.31/27.41

38.91

-∞

13

gi|304557842

Uncharacterized membrane protein YqjD [Edwardsiella tarda FL6-60]

YqjD

600

6.26/10.94

82.18

0.25 ± 0.04

Cellular processes

12

gi|269140157

iscU gene product [Edwardsiella tarda EIB202]: FeS cluster assembly scaffold

IscU

102

4.74/13.86

55.47

2.23 ± 0.62

Unknown function and hypothetical protein

2

gi|269139286

Hypothetical protein 1 [Edwardsiella tarda EIB202]

 

153

5.64/58.02

29.04

-∞

9

gi|269140100

Hypothetical protein 2 [Edwardsiella tarda EIB202]

 

318

5.33/33.70

54.90

3.21 ± 1.28

16e

NA

NA

NA

NA

NA

NA

5.21 ± 1.25

17

NA

NA

NA

NA

NA

NA

0.31 ± 0.05

18

NA

NA

NA

NA

NA

NA

2.94 ± 0.85

19

NA

NA

NA

NA

NA

NA

4.22 ± 1.77

20

NA

NA

NA

NA

NA

NA

0.32 ± 0.06

  1. aSpot ID represents the number on the 2-DE gels (Figure 5).
  2. bMOWSE score is -10 log (p), where p is the probability that the observed match is a random event. Based on the NCBInr database using the MASCOT searching program as MS/MS data. Scores greater than 65 are significant (p < 0.05).
  3. cNumber of amino acids spanned by the assigned peptides divided by the protein sequence length.
  4. dMean, the average protein abundance ratio for three paired samples. SD means the standard deviation of protein abundance ratios of one certain spot of three paired samples.
  5. eNot analyzed.