Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts

Pezoa, David; Blondel, Carlos J; Silva, Cecilia A; Yang, Hee-Jeong; Andrews-Polymenis, Helene; Santiviago, Carlos A; Contreras, Inés

doi:10.1186/1297-9716-45-2

Veterinary Research

Table 2 Primers used in this study

From: Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts

Primer	Sequence^a
Mutagenesis
SPI-6_T6SS_(H1 + P1)	AGGGTGTTTTTATACATCCTGTGAAGTAAAAAAAACCGTAGTGTAGGCTGGAGCTGCTTC
SPI-6_T6SS_(H2 + P2)	GTGAACATGGCACATTAATTTGAAGCAGCTCTCATCCGGTCATATGAATATCCTCCTTAG
SPI-6_OUT5	CCGAAGTGTATCTGGCGATGA
SD_∆phoN _(H1 + P1)	GTGAGTCTTTATGAAAAGTCGTTATTTAGTATTTTTTCTAGTGTAGGCTGGAGCTGCTTC
SD_∆phoN _(H2 + P2)	ACTTTCACCTTCAGTAATTAAGTTCGGGGTGATCTTCTTTCATATGAATATCCTCCTTAG
SD_∆phoN _OUT5	TTGCCTGATCCGGAGTGA
K1	CAGTCATAGCCGAATAGCCT
C3	CAGCTGAACGGTCTGGTTATAGG
VEX Capture
SeD_A0289_VEX_H1_U1	TTAACCGGGATCGGGACATGTTCAGCGCAGAAGCAGACTGGGCCACGTGGGCCGTGCACCTTAAGCTT
SeD_A0289_VEX_H2_U2	GAGGTTATTCATGTCAACAGGATTACGTTTCACACTGGAGGTGCAGGCTGGAGCTGCTTC
SeD_A0326_VEX_H1_D1	GGGGAGGTTGTGCGACGTTTGCATAATCCAGCAAGAACTGGGTTTAACGGTTGTGGACAACAAGCCAGGG
SeD_A0326_VEX_H2_D2	ACACAGGCCAGACTGATTATACAGGCATGAAAAAGCTCTCCAGGTCGACGTCCCATGGCCATTCGAATTC
SD_VC_OUT5	GCTCTAGA CCGGAGGGGTTATCTTTTCC
SD_VC_OUT3	GCTCTAGA TTGAAGCAGCTCTCATCCGG
5trfA	ACGTCCTTGTTGACGTGGAAAATGACCTTG
3trfA	CCGGAAGGCATACAGGCAAGAACTGATCG
SPI-6_OUT_DOWN	AAACGGGTCTATTTACAGGGGCAC
Tiling-PCR
1_T6SS_SPI-6_FOR	TTCAAGAAGTTCCACCGTCTATCG
1_T6SS_SPI-6_REV	ACCTGTTTGAGCTGCTACATACCAG
2_T6SS_SPI-6_FOR	CATTCAGTTCGCCGTCAAAGTG
2_T6SS_SPI-6_REV	CCGCTGCGAATTTTGTTATCG
3_T6SS_SPI-6_FOR	CCACGTTCTTCGGCATTACCAG
3_T6SS_SPI-6_REV	CGGTGTTGTAAACCAGATGCTCC
4_T6SS_SPI-6_FOR	AGACGCTGGCGAACACGATC
4_T6SS_SPI-6_REV	TAAGCACTGGCCGTAGCTCTGG
5_T6SS_SPI-6_FOR	GCAGCCATCCTTTGCACAAG
5_T6SS_SPI-6_REV	GGTTGTGTTATTGGCGGCTTC
6_T6SS_SPI-6_FOR	TATGCGATCAGGCGAACCTG
6_T6SS_SPI-6_REV	TCTTCCTGTAACCGGGTATCCAG
7_T6SS_SPI-6_FOR	GGTTGGATCAGGGACTGGATACC
7_T6SS_SPI-6_REV	CGTAACCCTCAACATCCTGCG
8_T6SS_SPI-6_FOR	AAAGCACCGGTGAATGTGGCTG
8_T6SS_SPI-6_REV	TCGGTGTGGTCATCCTTACGGG
9_T6SS_SPI-6_FOR	TGTCAGCACCAACAGTCGCC
9_T6SS_SPI-6_REV	CGCCCTTCGATAGAATCTGGC
10_T6SS_SPI-6_FOR	TAGTAGGGCCAGATTCTATCGAAGG
10_T6SS_SPI-6_REV	CCCTCCGGCTTTTACACATTATTC

^aItalics indicate the region that anneals to the 5′ or 3′ end of the antibiotic resistance cassette used for the mutagenesis. Underline indicates Xba I restriction sites used for cloning an internal region of homology to T6SS_SPI-6 into R995 plasmid.

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ISSN: 1297-9716

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