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Figure 5 | Veterinary Research

Figure 5

From: Myosins 1 and 6, myosin light chain kinase, actin and microtubules cooperate during antibody-mediated internalisation and trafficking of membrane-expressed viral antigens in feline infectious peritonitis virus infected monocytes

Figure 5

Role of actin in the internalisation of surface expressed viral antigens in FIPV-infected monocytes. (A) Quantification of the internalisation of surface expressed viral antigens in the presence of actin inhibitors. Results are given relatively to a control of untreated cells. Data are means and standard deviations of triplicate assays. The asterisk marks results that are significantly different from the untreated control (p < 0.05). (B) Confocal images of monocytes after internalisation in the presence of actin inhibitors. The activity of each inhibitor was tested with internalisation assays of fluorescent beads. In row 2, cortical actin was stained (red) to visualise whether or not the lamellipodia were closed around the beads. (C) Visualisation of actin dynamics (red) during antibody-induced internalisation of surface expressed viral antigens (green) in FIPV-infected monocytes at some time points after antibody addition. (D) A confocal image of an infected monocyte at 30 min post antibody addition during treatment with Latrunculin B and ML-7. All images show a single optical section through a monocyte and the scale bars represent 5 μm.

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Veterinary Research

ISSN: 1297-9716