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Figure 1 | Veterinary Research

Figure 1

From: Autophagic response in the Rabbit Hemorrhagic Disease, an animal model of virally-induced fulminant hepatic failure

Figure 1

Liver expression of the RHDV capsid protein VP60. A: Levels of mRNA analyzed by real-time PCR assay and normalized against β-Actin. Data are presented as percentage change from the control group. Values are expressed as means SEM (n = 6). B-C: Immunohistochemical labeling in hepatocytes (a) Control, (b) RHDV 12 hpi; (c) RHDV 18 hpi; (d) RHDV 24 hpi; (e) RHDV 30 hpi; (F) RHDV 36 hpi. Paraffin-embedded sections were immunostained with a VP60 antibody. Original magnification: 200×. The graph shows evolution of the percentage of positively labeled hepatocytes over time. Values are expressed as means S.E.M (n = 6). *P < 0.05, compared with Control. Image analysis was performed using the ImageJ software v3.91 [30].

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