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Table 4 Virus isolation, and mean scores for PRRSV-positive cells by in situ hybridization (ISH) and real-time PCR-positive numbers in litters from sows

From: Vaccination of sows against type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) before artificial insemination protects against type 2 PRRSV challenge but does not protect against type 1 PRRSV challenge in late gestation

Test Group n Lung LNa Heart Tonsil Thymus Liver Spleen
PRRSV 1 48 41b 39 31 40 43 18 30
(real-time PCR)    (1.3 ± 0.3)c (1.5 ± 0.4) (0.4 ± 0.5) (1.4 ± 0.6) (2.9 ± 0.6) (0.3 ± 0.3) (1.1 ± 0.4)
  2 51 6 5 4 4 7 0 4
(0.3 ± 0.1)* (0.4 ± 0.2)* (0.1 ± 0.3)* (0.4 ± 0.3)* (0.5 ± 0.3)* 0* (0.2 ± 0.3)*
  3 47 44 41 32 42 44 21 33
(1.5 ± 0.5) (1.8 ± 0.5) (0.5 ± 0.4) (1.5 ± 0.6) (3.2 ± 0.7) (0.3 ± 0.1) (1.0 ± 0.5)
4 48 42 40 28 39 45 19 35
(1.4 ± 0.2) (1.5 ± 0.7) (0.2 ± 0.2) (1.1 ± 0.3) (2.8 ± 0.4) (0.3 ± 0.3) (0.9 ± 0.4)
5 27 0 0 0 0 0 0 0
6 29 0 0 0 0 0 0 0
PRRSV 1 48 5.7 ± 1.9 13.5 ± 3.7 4.0 ± 0.9 13.1 ± 3.5 31.5 ± 6.9 2.4 ± 0.5 13.7 ± 2.3
(ISH) 2 51 2.4 ± 0.9* 4.5 ± 1.1* 0.4 ± 0.5* 4.5 ± 1.2* 6.5 ± 2.4* 0* 1.6 ± 0.7*
3 47 7.3 ± 1.7 16.7 ± 3.9 3.4 ± 1.2 15.2 ± 2.9 40.8 ± 4.8 1.9 ± 0.8 13.9 ± 2.4
4 48 7.1 ± 2.2 14.8 ± 1.9 2.6 ± 1.3 15.0 ± 3.4 39.9 ± 5.1 1.8 ± 0.7 13.5 ± 2.6
5 27 0 0 0 0 0 0 0
6 29 0 0 0 0 0 0 0
PRRSV 1 48 31 33 20 30 34 11 23
(Isolation) 2 51 0 0 0 0 0 0 0
3 47 34 36 21 29 37 14 27
4 48 33 35 20 26 35 12 27
5 27 0 0 0 0 0 0 0
  6 29 0 0 0 0 0 0 0
  1. aLN, inguinal lymph node.
  2. bNumber of positive fetuses.
  3. cMean values of the genomic copies of PRRSV RNA in fetal tissues (log10).
  4. *Significant (P < 0.05) difference between group 2 and group 4.