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Table 2 Detection of foetal death, foetal serology and PCR-detection of N. caninum in placenta and foetuses

From: Neospora caninum infection during early pregnancy in cattle: how the isolate influences infection dynamics, clinical outcome and peripheral and local immune responses

Group

 Foetus ref.

Foetal death (dpi)

IFAT titreb

Parasite ITS1 PCR detectiona

    

Placental tissues

Foetal tissues

    

Caruncle

Cotyledon

Brain

Liver

Heart

Lung

Muscle

Foetal fluidsc

1

F2849

34

32

5/5

5/5

4/5

3/3

3/3

3/3

3/3

2/2

(Nc-Spain7)

F3237

49

128

4/5

5/5

5/5

3/3

0/3

3/3

3/3

2/2

F3255

24

-

5/5

5/5

5/5

3/3

3/3

3/3

2/3

1/2

F9500 (1)*

34

16

5/5

5/5

5/5

3/3

3/3

3/3

3/3

2/2

F9500 (2)

34

16

4/5

5/5

5/5

3/3

3/3

3/3

3/3

2/2

F9522

34

32

4/5

5/5

5/5

3/3

3/3

3/3

3/3

2/2

F9516

27

-

5/5

4/5

5/5

3/3

3/3

3/3

3/3

2/2

2

F3236

44

N.D.

3/5

4/5

5/5

2/3

0/3

0/3

2/3

N.D.

(Nc-Spain8)

F3263

30

-

2/5

5/5

4/5

1/3

2/3

3/3

3/3

2/2

F9507

41

-

4/5

5/5

5/5

3/3

3/3

3/3

3/3

2/2

F9510

41

32

1/5

5/5

5/5

3/3

0/3

3/3

0/3

2/2

F9517

44

64

5/5

5/5

5/5

3/3

3/3

3/3

3/3

2/2

 

F9530

37

-

5/5

5/5

5/5

2/3

3/3

3/3

2/3

2/2

  1. aFractions represent the number of positive tissue samples/total number of samples tested by nested-ITS1 PCR.
  2. bIFAT titres assessed in thoracic fluids.
  3. cParasite detection in one aliquot of thoracic and abdominal foetal fluids.
  4. *Heifer H9500 was carrying twins. Both foetuses were independently analysed.
  5. N.D. Not evaluated.