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Table 2 Detection of foetal death, foetal serology and PCR-detection of N. caninum in placenta and foetuses

From: Neospora caninum infection during early pregnancy in cattle: how the isolate influences infection dynamics, clinical outcome and peripheral and local immune responses

Group  Foetus ref. Foetal death (dpi) IFAT titreb Parasite ITS1 PCR detectiona
     Placental tissues Foetal tissues
     Caruncle Cotyledon Brain Liver Heart Lung Muscle Foetal fluidsc
1 F2849 34 32 5/5 5/5 4/5 3/3 3/3 3/3 3/3 2/2
(Nc-Spain7) F3237 49 128 4/5 5/5 5/5 3/3 0/3 3/3 3/3 2/2
F3255 24 - 5/5 5/5 5/5 3/3 3/3 3/3 2/3 1/2
F9500 (1)* 34 16 5/5 5/5 5/5 3/3 3/3 3/3 3/3 2/2
F9500 (2) 34 16 4/5 5/5 5/5 3/3 3/3 3/3 3/3 2/2
F9522 34 32 4/5 5/5 5/5 3/3 3/3 3/3 3/3 2/2
F9516 27 - 5/5 4/5 5/5 3/3 3/3 3/3 3/3 2/2
2 F3236 44 N.D. 3/5 4/5 5/5 2/3 0/3 0/3 2/3 N.D.
(Nc-Spain8) F3263 30 - 2/5 5/5 4/5 1/3 2/3 3/3 3/3 2/2
F9507 41 - 4/5 5/5 5/5 3/3 3/3 3/3 3/3 2/2
F9510 41 32 1/5 5/5 5/5 3/3 0/3 3/3 0/3 2/2
F9517 44 64 5/5 5/5 5/5 3/3 3/3 3/3 3/3 2/2
  F9530 37 - 5/5 5/5 5/5 2/3 3/3 3/3 2/3 2/2
  1. aFractions represent the number of positive tissue samples/total number of samples tested by nested-ITS1 PCR.
  2. bIFAT titres assessed in thoracic fluids.
  3. cParasite detection in one aliquot of thoracic and abdominal foetal fluids.
  4. *Heifer H9500 was carrying twins. Both foetuses were independently analysed.
  5. N.D. Not evaluated.