Figure 4
From: Diacylated lipopeptide from Mycoplasma synoviae mediates TLR15 induced innate immune responses
Mycoplasma synoviae derived diacylated lipopeptide induces iNOS expression and NO production. A) Expression of the avian iNOS gene after infection of cells with Mycoplasma synoviae or treatment with diacylated lipopeptide. HD11 cells were infected with Mycoplasma synoviae (MS) or treated with 1 μM non-acylated peptide (NAP) or diacylated lipopeptide (MDLP) for 1 h, 6 h or 24 h and mRNA expression was analyzed by RT-qPCR. NC represents non-infected cells. Bars show mean ± S.E. (n = 4); ***, p < 0.001 compared to non-treated cells for Mycoplasma synoviae or NAP-treated cells at each time point, respectively. B) MDLP induces NO production in macrophages. HD11 cells were or treated with 0.5, 1 or 1.5 μM non-acylated peptide (NAP) or diacylated lipopeptide (MDLP) for 6 h or 24 h, and production of nitrite was measured by Griess assay. NC represents non-treated cells. LPS treated cells were used as positive control. Triangles represent increasing concentrations of NAP or MDLP. Bars show mean ± S.E. (n = 4); ***, p < 0.001 compared to non-treated cells for LPS or NAP-treated cells at concentration, respectively.